| Pan-sulfation of bile salts markedly increases hydrophilicity and essentially abolishes self- and hetero-association with lecithin. | |
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MedLine Citation:
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PMID: 8347684 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In chronic liver disease, partially and to a lesser extent completely (pan-)sulfated common bile salts are synthesized, yet little information is available concerning their physical-chemical characteristics. We studied solution properties of pan-sulfated common free, taurine and glycine-conjugated bile salts, and the interactions of taurodeoxycholate di-sulfate (TDC-S) with lecithin. By reverse-phase HPLC, pan-sulfated glycine and taurine-conjugated bile salts were very hydrophilic, with hydrophobic indices 1.7 to 2.5 units lower than their non-sulfated congeners. In contrast to non-sulfated species, pan-sulfated free and glycine-conjugated bile salts produced simple potentiometric titration curves without precipitation of bile salt below the pK'A of the carboxylic acids. By quasi-elastic light scattering, critical micellar concentrations of TDC-S fell from 28 mM in 0.15 M NaCl to 3 mM in 4.0 M NaCl, a value slightly higher than that of TDC. TDC-S formed very small micelles (hydrodynamic radii approx. 11A) that, in contrast to TDC, did not grow with increases in bile salt (7-66 mM) or NaCl (0.15-2.0 M) concentrations. TDC-S formed mixed micelles with lecithin in 0.15 M NaCl, but with a micellar zone drastically reduced compared with that of the non-sulfated congener. However, in 4 M NaCl, the micellar zone of TDC-S expanded and approached that of the non-sulfated parent compound. Therefore, under physiological conditions, pan-sulfation of common bile salts should largely eliminate their capacity to form mixed micelles with membrane lipids. |
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Authors:
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J M Donovan; I M Yousef; M C Carey |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 1182 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1993 Aug |
Date Detail:
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Created Date: 1993-09-15 Completed Date: 1993-09-15 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 37-45 Citation Subset: IM |
Affiliation:
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Department of Medicine, Harvard Medical School, Brigham and Women's Hospital, Boston, MA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bile Acids and Salts
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chemistry*,
metabolism Cholestasis / metabolism Glycine / chemistry Humans Hydrogen-Ion Concentration Liver Diseases / metabolism Membrane Lipids / metabolism Particle Size Phosphatidylcholines / chemistry* Potentiometry Solutions Sulfates / chemistry* Taurine / chemistry |
| Grant Support | |
ID/Acronym/Agency:
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DK 34854/DK/NIDDK NIH HHS; DK 36588/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Bile Acids and Salts; 0/Membrane Lipids; 0/Phosphatidylcholines; 0/Solutions; 0/Sulfates; 107-35-7/Taurine; 56-40-6/Glycine |
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