| Pan and sentinel lymph node visualization using a near-infrared fluorescent probe. | |
| | |
MedLine Citation:
|
PMID: 12926234 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
A number of different types of agents have been employed to aid in the visualization of lymph nodes, particularly the sentinel lymph node, and to decrease the tissue destruction associated with the diagnosis of nodal metastases. The current study was performed to see if a novel macromolecular near-infrared fluorescent (NIRF) probe could be used to visualize lymph nodes after intravenous administration (pan-node visualization) or subcutaneous administration (sentinel node visualization), and serve as method for guiding dissection with interventional radiologic and surgical procedures. Cy5.5-PGC, the near-infrared dye Cy5.5 coupled to a protected graft copolymer (PGC), was injected (i.v. or s.c.) into nude mice. Twenty-four hours later white light and NIRF images were obtained on (i) the live animal, (ii) a partially dissected animal, and (iii) tissue specimens. With Cy5.5-PGC administered intravenously, axillary nodes were visualized from outside a living mouse. With partial dissection, iliac and aortic nodes were visible as concentrated foci of high-intensity NIRF signals. With subcutaneous injection in the front extremity, axillary and brachial nodes draining the injection site were easily visualized. NIRF imaging provides a nonradioactive method of visualizing lymph nodes through layers of tissue that can be employed with intravenous or subcutaneous injection. |
| | |
Authors:
|
Lee Josephson; Umar Mahmood; Patrick Wunderbaldinger; Yi Tang; Ralph Weissleder |
Related Documents
:
|
3942974 - Epidermoid carcinoma of the anal canal. 10610624 - Guanylyl cyclase c messenger rna is a biomarker for recurrent stage ii colorectal cancer. 20395864 - Prognostic significance of the number of lymph nodes examined in colon cancer surgery: ... 12414344 - Lymphomatous superficial lymph nodes: limitations of physical examination for accurate ... 22951174 - Immunohistochemical expression of cd44s in renal cell carcinoma lacks independent progn... 18427894 - Transcatheter arterial chemoembolization for advanced hepatocellular carcinoma with inf... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Molecular imaging Volume: 2 ISSN: 1535-3508 ISO Abbreviation: Mol Imaging Publication Date: 2003 Jan |
Date Detail:
|
Created Date: 2003-08-20 Completed Date: 2003-10-23 Revised Date: 2011-04-07 |
Medline Journal Info:
|
Nlm Unique ID: 101120118 Medline TA: Mol Imaging Country: United States |
Other Details:
|
Languages: eng Pagination: 18-23 Citation Subset: IM |
Affiliation:
|
MGH/Harvard Medical School, Building 149, 13th Street, Charlestown, MA 02129, USA. ljosephson@partners.org |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Carbocyanines / diagnostic use, pharmacokinetics Fluorescent Dyes / diagnostic use*, pharmacokinetics Lymph Nodes / anatomy & histology* Mice Mice, Nude Sentinel Lymph Node Biopsy Spectroscopy, Near-Infrared / methods* |
| Chemical | |
Reg. No./Substance:
|
0/CY5.5 cyanine dye; 0/Carbocyanines; 0/Fluorescent Dyes |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Image-guided control of transgene expression based on local hyperthermia.
Next Document: A novel method of imaging lysosomes in living human mammary epithelial cells.