| Palmitoleic (16:1 cis-9) and cis-Vaccenic (18:1 cis-11) Acid Alter Lipogenesis in Bovine Adipocyte Cultures. | |
| | |
MedLine Citation:
|
PMID: 23077002 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Our objectives were to: (1) confirm elongation products of palmitoleic acid (16:1 cis-9) elongation in vitro using stable isotopes and (2) evaluate if exogenous supplementation of palmitoleic acid, elongation products, or both are responsible for decreased desaturation and lipogenesis rates observed with palmitoleic acid supplementation in bovine adipocytes. Stromal vascular cultures were isolated from adipose tissue of two beef carcasses, allowed to reach confluence, held for 2 days, and differentiated with a standard hormone cocktail (day 0). On day 2, secondary differentiation media containing 1 of 4 fatty acid treatments [0 μM fatty acid (control), or 150 μM palmitic (16:0), palmitoleic, or cis-vaccenic (18:1 cis-11)] was added for 4 days. On day 6, cells were incubated with [(13)C] 16:1, [(13)C] 2, or [(13)C] 18:0 to estimate elongation, lipogenic, and desaturation rates using gas chromatography-mass spectrometry. Enrichment of [(13)C] 18:1 cis-11 confirmed 18:1 cis-11 is an elongation product of 16:1. Additionally, [(13)C] label was seen in 20:1 cis-13 and cis-9, cis-11 CLA. Synthesis of [(13)C] 16:0 from [(13)C] 2 was reduced (P < 0.05) in palmitoleic acid and cis-vaccenic acid-treated compared with control cells following 36 h incubation. By 12 h of [(13)C] 18:0 incubation, cells supplemented with palmitoleic acid had reduced (P < 0.05) [(13)C] 18:1 cis-9 compared with all other treatments. Gene expression and fatty acid results support isotopic data for lipogenesis and desaturation. Therefore, palmitoleic acid is actively elongated in vitro and its elongation product, cis-vaccenic acid, can also reduce lipogenesis. However, inhibition of desaturation can be directly attributed to palmitoleic acid and not its elongation products, 18:1 cis-11 or 20:1 cis-13. |
| | |
Authors:
|
T A Burns; A K G Kadegowda; S K Duckett; S L Pratt; T C Jenkins |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-10-18 |
Journal Detail:
|
Title: Lipids Volume: - ISSN: 1558-9307 ISO Abbreviation: Lipids Publication Date: 2012 Oct |
Date Detail:
|
Created Date: 2012-10-18 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0060450 Medline TA: Lipids Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Department of Animal and Veterinary Sciences, Clemson University, Clemson, SC, 29634-0311, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Key Fatty Acid Combinations Define Vascular Smooth Muscle Cell Proliferation and Viability.
Next Document: Cytotoxic Petrosiacetylenes from the Marine Sponge Petrosia sp.