| Palmitate activates insulin signaling pathway in pancreatic rat islets. | |
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MedLine Citation:
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PMID: 19287336 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: To investigate the action of palmitate on insulin receptor (IR) signaling pathway in rat pancreatic islets. The following proteins were studied: IR substrate-1 and -2 (IRS1 and IRS2), phosphatidylinositol 3-kinase, extracellular signal-regulated protein kinase-1 and -2 (ERK1/2), and signal transducer and activator of transcription 3 (STAT3). METHODS: Immunoblotting and immunoprecipitation assays were used to evaluate the phosphorylation states of IRS1 and IRS2 (tyrosine [Tyr]), ERK1/2 (threonine 202 [Thr202]/Tyr204), and STAT3 (serine [Ser727]). RESULTS: The exposure of rat pancreatic islets to 0.1-mmol/L palmitate for up to 30 minutes produced a significant increase of Tyr phosphorylation in IRS2 but not in IRS1. The association of phosphatidylinositol 3-kinase with IRS2 was also upregulated by palmitate. Exposure to 5.6-mmol/L glucose caused a gradual decrease in ERK1/2 (Thr202/Tyr204) and STAT3 (serine [Ser727]) phosphorylations after 30-minute incubation. The addition of palmitate (0.1 mmol/L), associated with 5.6-mmol/L glucose, abolished these latter effects of glucose after 15-minute incubation. CONCLUSIONS: Palmitate at physiological concentration associated with 5.6-mmol/L glucose activates IR signaling pathway in pancreatic beta cells. |
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Authors:
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Maria Fernanda R Graciano; Tatiane C A Nogueira; Carla R O Carvalho; Rui Curi; Angelo R Carpinelli |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Pancreas Volume: 38 ISSN: 1536-4828 ISO Abbreviation: Pancreas Publication Date: 2009 Jul |
Date Detail:
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Created Date: 2009-06-25 Completed Date: 2009-09-25 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 8608542 Medline TA: Pancreas Country: United States |
Other Details:
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Languages: eng Pagination: 578-84 Citation Subset: IM |
Affiliation:
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Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil. mafe@icb.usp.br |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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1-Phosphatidylinositol 3-Kinase
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metabolism Animals Caspase 3 / metabolism Cell Line Female Glucose / pharmacology Immunoblotting Immunoprecipitation Insulin Receptor Substrate Proteins / metabolism Islets of Langerhans / drug effects*, metabolism Macrophages / cytology, drug effects, enzymology Mitogen-Activated Protein Kinase 1 / metabolism Mitogen-Activated Protein Kinase 3 / metabolism Palmitates / pharmacology* Phosphorylation / drug effects Rats Receptor, Insulin / metabolism* STAT3 Transcription Factor / metabolism Signal Transduction / drug effects* Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Insulin Receptor Substrate Proteins; 0/Irs1 protein, rat; 0/Irs2 protein, rat; 0/Palmitates; 0/STAT3 Transcription Factor; 0/Stat3 protein, rat; 50-99-7/Glucose; EC 2.7.1.137/1-Phosphatidylinositol 3-Kinase; EC 2.7.10.1/Receptor, Insulin; EC 2.7.11.24/Mitogen-Activated Protein Kinase 1; EC 2.7.11.24/Mitogen-Activated Protein Kinase 3; EC 3.4.22.-/Caspase 3 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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