Document Detail


Paeonol Prevents Excitotoxicity in Rat Pheochromocytoma PC12 Cells via Downregulation of ERK Activation and Inhibition of Apoptosis.
MedLine Citation:
PMID:  21509715     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Paeonol, an active component of Moutan Cortex, has been recognized as a potential neuroprotective drug. In the present study, an injury model based on glutamate-induced cell death of rat pheochromocytoma cells was used to investigate the neuroprotective potential of paeonol and its mechanism of action. Our findings showed that paeonol dose-dependently prevented glutamate-induced cell death as evidenced by cell viability, lactate dehydrogenase release, and trypan blue exclusion. In addition, flow cytometry of propidium iodide-stained cells revealed that paeonol pretreatment reduced the level of glutamate-induced apoptosis in pheochromocytoma cells. Paeonol was also able to decrease the glutamate-induced injury of mitochondria by normalization of mitochondrial membrane potential and cytochrome c release. The glutamate-induced activity of caspase-3 and p-ERK were dose-dependently reduced by paeonol pretreatments. Taken together, our data suggest that paeonol develops its neuroprotective effect against glutamate neurotoxicity through inhibition of the apoptotic signaling pathway and upregulation of the p-ERK pathway.
Authors:
Xuncui Wang; Guoqi Zhu; Shu Yang; Xiu Wang; Hui Cheng; Fei Wang; Xiaoxiang Li; Qinglin Li
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-4-20
Journal Detail:
Title:  Planta medica     Volume:  -     ISSN:  1439-0221     ISO Abbreviation:  -     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-4-21     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0066751     Medline TA:  Planta Med     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© Georg Thieme Verlag KG Stuttgart · New York.
Affiliation:
Key Laboratory of Xin'an Medicine, Ministry of Education, Anhui University of Traditional Chinese Medicine, Hefei, China.
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