Document Detail

Paeoniflorin inhibits growth of human colorectal carcinoma HT 29 cells in vitro and in vivo.
MedLine Citation:
PMID:  22326807     Owner:  NLM     Status:  Publisher    
The aim of the therapy of human malignancies is the inhibition of cell proliferation and/or induction of apoptosis. In present experiment, we investigated the in vitro and in vivo anticancer effects and associated mechanisms of paeoniflorin (PF), isolated from the paeony root, against colorectal cancer. In vitro, cell growth assay obviously showed the inhibition of tumor cell growth in a dose-dependent manner. Flow cytometry analysis showed that PF could mainly have the cell cycle arrest at G1, which is associated with DNA damage and activation of p53/14-3-3 zeta (ζ). The pro-apoptotic effect of PF was demonstrated by Annexin V-PI staining, and activation of caspase-3 and caspase-9 by Western immunoblotting. In vivo, the results showed that positive cells of PCNA in PF and docetaxel-treated group was decreased to 30% and 15% compared with control group of tumors, respectively. But apoptosis cells in PF- and docetaxel treated groups studied by TUNEL is increased to 40±1.2% and 30±1.5% compared with 24±2.3% in negative control, respectively. Furthermore, the efficiency of tumor-bearing mice treated by PF was superior to docetaxel in vivo. Overall, PF may be an effective chemopreventive agent against colorectal cancer HT29, and the mechanism could be mediated via an regulation of p53/14-3-3ζ.
He Wang; Hong Zhou; Cheng-Xi Wang; Yue-Shan Li; Hong-Yan Xie; Jian-Dong Luo; Yi Zhou
Related Documents :
22895077 - Reporting of preclinical tumor-graft cancer therapeutic studies.
22848167 - A novel lipid-based nanomicelle of docetaxel: evaluation of antitumor activity and biod...
22757197 - We-c-bra-07: assessment of heterogeneous treatment response in patients with multiple s...
3518597 - Sensitization of p388 murine leukemia cells to hydroxyurea cytotoxicity by hydrophobic ...
17957467 - 2-meoe2bismate and 2-ete2bismate induce cell cycle arrest and apoptosis in breast cance...
8062267 - Effect of 1,19-bis(ethylamino)-5,10,15-triazanonadecane on human tumor xenografts.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-2-2
Journal Detail:
Title:  Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association     Volume:  -     ISSN:  1873-6351     ISO Abbreviation:  -     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-2-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8207483     Medline TA:  Food Chem Toxicol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012. Published by Elsevier Ltd.
Department of Pharmaceutical Science, Guangzhou Medical University, Guangzhou 510182, China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Evaluation of hepatotoxicity and oxidative stress in rats treated with tert-butyl hydroperoxide.
Next Document:  Health risk assessment of polycyclic aromatic hydrocarbons and heavy metals via dietary intake of ve...