Document Detail

Paclitaxel inhibits expression of heat shock protein 27 in ovarian and uterine cancer cells.
MedLine Citation:
PMID:  15304155     Owner:  NLM     Status:  MEDLINE    
The purpose of the study was to determine whether paclitaxel inhibits the expression of heat shock protein 27 (HSP27) in two gynecologic cancer cell lines compared with other antineoplastic agents having different cytotoxic mechanisms. BG-1 ovarian cancer cells and HeLa uterine cancer cells were treated with a tubulin depolymerization inhibitor (paclitaxel), a topoisomerase-II inhibitor (etoposide), and two tubulin polymerization inhibitors (colcemid and vincristine). Cell kills were evaluated by counting the number of cells. Propidium iodide staining and flow cytometric analysis were applied for the determination of cell-cycle perturbation. HSP27 was stained by the indirect immunofluorescence technique and analyzed with a flow cytometer. In both BG-1 and HeLa cells, growth arrest and G2 / M accumulation were dependent on the dose of each cytotoxic agent. There were positive correlations between HSP27 overexpression and growth arrest and G2 / M accumulation when the cell lines were treated with etoposide, colcemid, or vincristine, but not with paclitaxel. Paclitaxel completely inhibited the expression of HSP27. The results of this study indicated that paclitaxel may possess unique mechanisms able to overcome drug resistance by inhibiting HSP27 expression.
Y Tanaka; K Fujiwara; H Tanaka; K Maehata; I Kohno
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of gynecological cancer : official journal of the International Gynecological Cancer Society     Volume:  14     ISSN:  1048-891X     ISO Abbreviation:  Int. J. Gynecol. Cancer     Publication Date:    2004 Jul-Aug
Date Detail:
Created Date:  2004-08-12     Completed Date:  2004-11-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9111626     Medline TA:  Int J Gynecol Cancer     Country:  United States    
Other Details:
Languages:  eng     Pagination:  616-20     Citation Subset:  IM    
Department of Obstetrics and Gynecology, Kawasaki Medical School, Kurashiki-City, Japan.
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MeSH Terms
Adenocarcinoma / drug therapy,  metabolism
Antineoplastic Agents / therapeutic use*
Cell Line, Tumor
Demecolcine / therapeutic use
Etoposide / therapeutic use
Heat-Shock Proteins / biosynthesis*
Ovarian Neoplasms / drug therapy*,  metabolism
Paclitaxel / therapeutic use*
Uterine Neoplasms / drug therapy*,  metabolism
Vincristine / therapeutic use
Reg. No./Substance:
0/Antineoplastic Agents; 0/Heat-Shock Proteins; 33069-62-4/Paclitaxel; 33419-42-0/Etoposide; 477-30-5/Demecolcine; 57-22-7/Vincristine

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