| Packed blood cells stored in AS-5 become proinflammatory during storage. | |
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MedLine Citation:
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PMID: 19374730 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Studies have shown that packed blood cells (PBCs) stored in AS-1 (Adsol, Baxter) and AS-3 (Nutricel, Medsep Corp.) accumulate proinflammatory substances, which may contribute to increased complications from allogeneic blood transfusion. This study assessed whether supernates from PBCs stored in AS-5 (Optisol, Terumo Corp.) prime neutrophils (PMNs), activate platelets (PLTs), and accumulate proinflammatory cytokines and PMN granule constituents. STUDY DESIGN AND METHODS: PBC units were prepared in AS-5 from nonleukoreduced (NLR) and leukoreduced (LR) whole-blood units and stored at 4 degrees C. Supernates from samples of PBCs collected at various storage times were analyzed by multiplex enzyme-linked immunosorbent assay for proinflammatory cytokines and myeloperoxidase (MPO) and were incubated with type-matched blood, which was assessed by flow cytometry for expression of CD11b on PMNs, CD62P on PLTs, and formation of PMN-PLT aggregates. RESULTS: Supernates from NLR PBCs stored for at least 14 days elevated CD11b expression on PMNs and the number of PMN-PLT aggregates compared to supernates from collection day PBCs. The magnitude of these effects correlated with storage age. Supernates from LR PBCs did not elicit these responses. Expression of CD62P on PLTs was not affected by supernates from either NLR or LR PBCs. Levels of interleukin (IL)-1beta, IL-6, IL-8, IL-18, NAP-2, MCP-1, RANTES, and MPO were elevated in supernates from 28- and 42-day NLR units. Tumor necrosis factor alpha and MIP-1alpha did not increase, and cytokine levels in LR PBC units did not increase. CONCLUSION: Units of NLR PBCs stored in AS-5 become increasingly proinflammatory as a function of storage time. Leukoreduction prevents this change. |
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Authors:
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Steve J McFaul; Jason B Corley; Craig W Mester; Jayasree Nath |
Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. Date: 2009-04-03 |
Journal Detail:
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Title: Transfusion Volume: 49 ISSN: 1537-2995 ISO Abbreviation: Transfusion Publication Date: 2009 Jul |
Date Detail:
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Created Date: 2009-07-15 Completed Date: 2009-07-31 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0417360 Medline TA: Transfusion Country: United States |
Other Details:
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Languages: eng Pagination: 1451-60 Citation Subset: IM |
Affiliation:
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Department of Blood Research, Division of Military Casualty Research, Walter Reed Army Institute of Research, 503 Robert Grant Avenue, Silver Spring, MD 20910, USA. steve.mcfaul@us.army.mil |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antigens, CD11b
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metabolism Blood Cells / immunology*, metabolism* Blood Platelets / metabolism Blood Preservation* Chemokine CCL2 / metabolism Chemokine CCL5 / metabolism Enzyme-Linked Immunosorbent Assay Flow Cytometry Humans Interleukin-18 / metabolism Interleukin-6 / metabolism Interleukin-8 / metabolism Neutrophils / metabolism Nuclear Proteins / metabolism P-Selectin / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD11b; 0/CCL2 protein, human; 0/Chemokine CCL2; 0/Chemokine CCL5; 0/ITGAM protein, human; 0/Interleukin-18; 0/Interleukin-6; 0/Interleukin-8; 0/NAP1L4 protein, human; 0/Nuclear Proteins; 0/P-Selectin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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