Document Detail


PUFAs enhance oxidative stress and apoptosis in tumour cells exposed to hypericin-mediated PDT.
MedLine Citation:
PMID:  20714672     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Since many studies have suggested the impact of dietary polyunsaturated fatty acids on cancer progression and prognosis, there is an assumption of possible pre-sensitizing effects of their application in combined treatment. The present work evaluates modulation of photodynamic therapy (PDT) with hypericin by pre-treatment with n-3 and n-6 fatty acids in HT-29 and HeLa tumour cells. We observed stimulation of cytotoxic effects by docosahexaenoic acid (n-3) and arachidonic acid (n-6) in several stages of action in both cell lines. Treatment with either fatty acids or photodynamic therapy alone induced apoptosis in a dose- and time-dependent manner; however the effect was even more striking in mutual combination applied as pre-treatment with fatty acids prior to photodynamic therapy. Moreover, the combination also induced changes in membrane lipid composition leading to alteration in cell membrane fluidity. Increased toxicity of combined treatment was also confirmed by the presence of oxidative stress demonstrated by ROS production, RNS accumulation and increased presence of lipoperoxides. In conclusion, we suggest that pre-treatment with polyunsaturated fatty acids may contribute to cytotoxic effects induced by photodynamic therapy with hypericin.
Authors:
Martin Kello; Jaromír Mikes; Rastislav Jendzelovský; Ján Koval; Peter Fedorocko
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-08-12
Journal Detail:
Title:  Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology     Volume:  9     ISSN:  1474-9092     ISO Abbreviation:  Photochem. Photobiol. Sci.     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-08-25     Completed Date:  2010-12-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101124451     Medline TA:  Photochem Photobiol Sci     Country:  England    
Other Details:
Languages:  eng     Pagination:  1244-51     Citation Subset:  IM    
Affiliation:
Institute of Biology and Ecology, Faculty of Science, P. J. Safárik University in Kosice, Moyzesova 11, 040 01, Kosice, Slovakia.
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MeSH Terms
Descriptor/Qualifier:
Antineoplastic Agents / therapeutic use*
Apoptosis*
Arachidonic Acid / toxicity
Cell Line, Tumor
Colonic Neoplasms / drug therapy
Docosahexaenoic Acids / toxicity
Fatty Acids, Unsaturated / toxicity*
HT29 Cells
Humans
Membrane Fluidity
Oxidative Stress*
Perylene / analogs & derivatives*,  therapeutic use
Photochemotherapy
Chemical
Reg. No./Substance:
0/Antineoplastic Agents; 0/Fatty Acids, Unsaturated; 198-55-0/Perylene; 25167-62-8/Docosahexaenoic Acids; 506-32-1/Arachidonic Acid; 548-04-9/hypericin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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