| PUB-NChIP - "in vivo biotinylation" approach to study chromatin in proximity to a protein of interest. | |
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MedLine Citation:
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PMID: 23038767 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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We have developed an approach termed PUB-NChIP (Proximity Utilizing Biotinylation with Native ChIP) to purify and study protein composition of chromatin in the proximity to a nuclear protein of interest. It is based on coexpression of a) a protein of interest, fused with the bacterial biotin ligase BirA together with b) a histone fused to BAP biotin acceptor peptide, which is specifically biotinylated by BirA fusion in the proximity of the protein of interest. Using RAD18 protein as a model, we demonstrate that the RAD18-proximal chromatin is enriched in some H4 acetylated species. Moreover, the Rad18-proximal chromatin containing a replacement histone H2AZ has a different pattern of H4 acetylation. Finally, biotin pulse-chase experiments show that H4 acetylation pattern starts to resemble acetylation pattern of total H4 after the proximity of chromatin to Rad18 has been lost. |
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Authors:
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Muhammad Shoaib; Arman Kulyyassov; Chloe Robin; Kinga Winczura; Pavel Tarlykov; Emmanuelle Despras; Patricia Kannouche; Erlan Ramanculov; Marc Lipinski; Vasily Ogryzko |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-10-4 |
Journal Detail:
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Title: Genome research Volume: - ISSN: 1549-5469 ISO Abbreviation: Genome Res. Publication Date: 2012 Oct |
Date Detail:
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Created Date: 2012-10-5 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9518021 Medline TA: Genome Res Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Institut Gustave Roussy, CNRS UMR8126, Universite Paris-Sud XI; |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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