| PTHrP increases RANKL expression by stromal cells from giant cell tumor of bone. | |
| | |
MedLine Citation:
|
PMID: 22102368 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Giant cell tumor of bone (GCT) presents with numerous osteoclast-like multinucleated giant cells that are principally responsible for the extensive bone resorption by the tumor. Although the precise etiology of GCT remains uncertain, the accumulation of giant cells is partially due to the high expression of the receptor activator of nuclear factor-κB ligand (RANKL) from the neoplastic stromal cells. Here, we have investigated whether parathyroid hormone-related protein (PTHrP) plays a role in the pathogenesis of GCT. Immunohistochemistry results revealed PTHrP expression in the stromal cells of the tumor, and that its receptor, the parathyroid hormone type 1 receptor (PTH1R), is expressed by both the stromal cells and giant cells. PCR and Western blot analyses confirmed the expression of PTHrP and PTH1R by isolated stromal cells from five patients presenting with GCT. Treatment of GCT stromal cells with varying concentrations of PTHrP (1-34) significantly increased both RANKL gene expression and the number of multinucleated cells formed from RAW 264.7 cells in co-culture experiments, whereas inhibition of PTHrP with a neutralizing antibody decreased RANKL gene expression. These results suggest that PTHrP is expressed within GCT by the stromal cells and can contribute to the abundant RANKL expression and giant cell formation within the tumor. © 2011 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res. |
| | |
Authors:
|
Robert W Cowan; Gurmit Singh; Michelle Ghert |
Related Documents
:
|
22122438 - The latency-associated nuclear antigen, a multifunctional protein central to kaposi's s... 21570988 - Characterization of microvascular endothelial cells isolated from the dermis of adult m... 8735068 - Generators of the brainstem auditory evoked potential in cat. iii: identified cell popu... 21833718 - Targeting glucosylceramide synthase sensitizes imatinib-resistant chronic myeloid leuke... 12205688 - Neuroprotective and restorative effects of intrastriatal grafting of encapsulated gdnf-... 23195878 - Studies on the mechanism of cell elongation in blepharisma japonicum i. a physiological... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-11-18 |
Journal Detail:
|
Title: Journal of orthopaedic research : official publication of the Orthopaedic Research Society Volume: - ISSN: 1554-527X ISO Abbreviation: - Publication Date: 2011 Nov |
Date Detail:
|
Created Date: 2011-11-21 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8404726 Medline TA: J Orthop Res Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Copyright © 2011 Orthopaedic Research Society. |
Affiliation:
|
Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario, Canada; Juravinski Cancer Centre, 699 Concession Street, Hamilton, Ontario, Canada L8V 5C2. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Polypropylene-containing synthetic mesh devices in soft tissue repair: A meta-analysis.
Next Document: Structural basis for extracellular interactions between calcitonin receptor-like receptor and recept...