Document Detail


PTEN down regulates AP-1 and targets c-fos in human glioma cells via PI3-kinase/Akt pathway.
MedLine Citation:
PMID:  17235455     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The continual activation of signaling cascades results in dramatic consequences that include loss of cellular growth control and neoplastic transformation. We show here that phosphoinositide 3-kinase and its mediator Akt was constitutively activated in glioma and that this might be due to the aberrant expression of their natural antagonist PTEN. The PTEN (phosphatase and tensin homologue deleted on chromosome ten) tumor suppressor gene modulates cell growth and survival through mechanisms that are incompletely understood. In this study, we investigated the possibility that PTEN mediates its effects through modulation of transcription factor AP-1, which is in part due to decrease in c-fos expression which was dependent on PI3kinase activity. Consistent with a reduction in the c-fos levels, an AP-1 dependent reporter gene was poorly induced in the PTEN expressing cell lines. In contrast to its effect on c-fos, PTEN did not affect the expression of c-Jun and other fos family members. We also show that the effect of PTEN on c-fos expression was due to its ability to antagonize PI3-kinase and could be mimicked by the expression of dominant negative Akt mutant. Taken together, these data indicate that the aberrant expression of PTEN contributes to the activation of the PI3kinase/Akt pathway and its transcription factor mediators in glioma. We conclude that the ectopic expression of PTEN down regulates the proliferation of glioma cells through the suppression of AP-1 and that this target might be essential for its central role in the growth and survival of glioma cancer cells.
Authors:
Dimpy Koul; Ruijun Shen; Shishir Shishodia; Yasanuri Takada; Krishna P Bhat; Shrikanth A G Reddy; Bharat B Aggarwal; W K Alfred Yung
Related Documents :
23509325 - Uncoupling rara transcriptional activation and degradation clarifies the bases for apl ...
18678645 - Csig inhibits pten translation in replicative senescence.
22747515 - Quantitative expression analysis of the apoptosis-related gene, bcl2l12, in head and ne...
22719865 - The adenomatous polyposis coli protein contributes to normal compaction of mitotic chro...
22899305 - Kiss1 expression in human female adipose tissue.
8944685 - Regulation and function of extracellular matrix intestinal epithelial restitution in vi...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2007-01-18
Journal Detail:
Title:  Molecular and cellular biochemistry     Volume:  300     ISSN:  0300-8177     ISO Abbreviation:  Mol. Cell. Biochem.     Publication Date:  2007 Jun 
Date Detail:
Created Date:  2007-06-01     Completed Date:  2007-09-25     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0364456     Medline TA:  Mol Cell Biochem     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  77-87     Citation Subset:  IM    
Affiliation:
Department of Neuro-Oncology, The University of Texas M. D. Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, TX 77030, USA. Dkoul@mdanderson.org
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
1-Phosphatidylinositol 3-Kinase / metabolism*
Cell Line, Tumor
Down-Regulation* / drug effects
Enzyme Activation / drug effects
Gene Expression Regulation, Neoplastic / drug effects
Genes, Reporter
Glioma / enzymology*,  genetics,  pathology
Humans
PTEN Phosphohydrolase / metabolism*
Proto-Oncogene Proteins c-akt / metabolism*
Proto-Oncogene Proteins c-fos / genetics,  metabolism*
Signal Transduction / drug effects
Transcription Factor AP-1 / metabolism*
Transcriptional Activation / drug effects
Tumor Necrosis Factor-alpha / pharmacology
Grant Support
ID/Acronym/Agency:
R01 CA 056041/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Proto-Oncogene Proteins c-fos; 0/Transcription Factor AP-1; 0/Tumor Necrosis Factor-alpha; EC 2.7.1.137/1-Phosphatidylinositol 3-Kinase; EC 2.7.11.1/Proto-Oncogene Proteins c-akt; EC 3.1.3.48/PTEN protein, human; EC 3.1.3.67/PTEN Phosphohydrolase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Prediction of protein subcellular localization by support vector machines using multi-scale energy a...
Next Document:  An evidence-based approach to the treatment of thyroid lymphoma.