| [PSI(+) ] prion variant establishment in yeast. | |
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MedLine Citation:
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PMID: 22998111 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Differences in the clinical pathology of mammalian prion diseases reflect distinct heritable conformations of aggregated PrP proteins, called prion strains. Here, using the yeast [PSI(+) ] prion, we examine the de novo establishment of prion strains (called variants in yeast). The [PSI(+) ] prion protein, Sup35, is efficiently induced to take on numerous prion variant conformations following transient overexpression of Sup35 in the presence of another prion, e.g. [PIN(+) ]. One hypothesis is that the first [PSI(+) ] prion seed to arise in a cell causes propagation of only that seed's variant, but that different variants could be initiated in different cells. However, we now show that even within a single cell, Sup35 retains the potential to fold into more than one variant type. When individual cells segregating different [PSI(+) ] variants were followed in pedigrees, establishment of a single variant phenotype generally occurred in daughters, granddaughters or great-granddaughters - but in 5% of the pedigrees cells continued to segregate multiple variants indefinitely. The data are consistent with the idea that many newly formed prions go through a maturation phase before they reach a single specific variant conformation. These findings may be relevant to mammalian PrP prion strain establishment and adaptation. |
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Authors:
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Jaya Sharma; Susan W Liebman |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-9-24 |
Journal Detail:
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Title: Molecular microbiology Volume: - ISSN: 1365-2958 ISO Abbreviation: Mol. Microbiol. Publication Date: 2012 Sep |
Date Detail:
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Created Date: 2012-9-24 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8712028 Medline TA: Mol Microbiol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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© 2012 Blackwell Publishing Ltd. |
Affiliation:
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Department of Biological Sciences, University of Illinois at Chicago, Chicago, IL, 60607, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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