Document Detail


PPARalpha agonists clofibrate and gemfibrozil inhibit cell growth, down-regulate hCG and up-regulate progesterone secretions in immortalized human trophoblast cells.
MedLine Citation:
PMID:  15194003     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We studied effects of PPARalpha agonists clofibric acid and gemfibrozil on cell growth and functions of immortalized human extravillous trophoblast cells. Levels of DNA and protein gradually increased during incubation for 4 days. Gemfibrozil (>0.25mM) and clofibric acid (2.5mM) suppressed the rate of increase in DNA and protein. Specific activities of fatty acyl-CoA oxidase and catalase were increased to about 1.2-2.0 times the control value by 0.05mM gemfibrozil and 1.0 and 2.5mM clofibric acid after incubation for 3 days. Acid phosphatase activity showed a small increase in response to both agents, but esterase activity changed little. The secretion of progesterone from the cells into the medium was increased by 0.25mM gemfibrozil and 1.0 and 2.5mM clofibric acid after incubation for 3 days, but that of human chorionic gonadotropin (hCG) was decreased by 0.35mM gemfibrozil and 2.5mM clofibric acid. The specific activity of lactate dehydrogenase in the cells was hardly changed at all after incubation for 3 days. These results suggest that gemfibrozil and clofibric acid inhibit the proliferation of trophoblast cells. Cell metabolism is probably affected by both agents. The two agents may down-regulate hCG and up-regulate progesterone secretions.
Authors:
Fumie Hashimoto; Yoshinobu Oguchi; Mieko Morita; Kikumi Matsuoka; Satoru Takeda; Masayuki Kimura; Hidenori Hayashi
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochemical pharmacology     Volume:  68     ISSN:  0006-2952     ISO Abbreviation:  Biochem. Pharmacol.     Publication Date:  2004 Jul 
Date Detail:
Created Date:  2004-06-14     Completed Date:  2004-08-02     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0101032     Medline TA:  Biochem Pharmacol     Country:  England    
Other Details:
Languages:  eng     Pagination:  313-21     Citation Subset:  IM    
Affiliation:
Department of Pathological Biochemistry, Faculty of Pharmaceutical Sciences, Josai University, Keyakidai, Sakado, Saitama 350-0295, Japan. hasimoto@josai.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Anticholesteremic Agents / pharmacology
Cell Division / drug effects
Chorionic Gonadotropin / metabolism*
Clofibrate / pharmacology*
Dose-Response Relationship, Drug
Down-Regulation
Drug Interactions
Gemfibrozil / pharmacology*
Humans
L-Lactate Dehydrogenase / metabolism
Peroxisomes / physiology
Progesterone / secretion*
Receptors, Cytoplasmic and Nuclear / agonists*
Subcellular Fractions
Transcription Factors / agonists*
Trophoblasts / drug effects*,  metabolism
Tumor Cells, Cultured
Up-Regulation
Chemical
Reg. No./Substance:
0/Anticholesteremic Agents; 0/Chorionic Gonadotropin; 0/Receptors, Cytoplasmic and Nuclear; 0/Transcription Factors; 25812-30-0/Gemfibrozil; 57-83-0/Progesterone; 637-07-0/Clofibrate; EC 1.1.1.27/L-Lactate Dehydrogenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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