Document Detail


PPAR-gamma expression modulates insulin sensitivity in C2C12 skeletal muscle cells.
MedLine Citation:
PMID:  15504754     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Peroxisome proliferator-activated receptor-gamma (PPAR-gamma) expression is very low in skeletal muscle cells, which is one of the most important target tissues for insulin and plays a predominant role in glucose homeostasis. It has recently been shown that muscle-specific PPAR-gamma deletion in mouse causes insulin resistance. However, it is likely that the observed effects might be due to secondary interaction in whole animal. The aim of the study was to explore the role of muscle PPAR-gamma in insulin sensitivity. We stably transfected C2C12 skeletal muscle cells with plasmids containing sense or antisense constructs of PPAR-gamma and examined the effect of modulation of PPAR-gamma expression in terms of glucose uptake. Effect was also examined in insulin-resistant C2C12 skeletal muscle cells. In transfected C2C12 cell line, the inhibition of PPAR-gamma expression (23.0 +/-0.005%) was observed to induce insulin resistance as determined by functional assessment of 2-deoxyglucose incorporation. Overexpression of PPAR-gamma (28.5 +/- 0.008%) produced an additional effect on insulin (100 nM) and Pioglitazone (50 microM), resulting in 42.7 +/- 3.5% increase in glucose uptake as against 29.2+/-2.8% in wild-type C2C12 skeletal muscle cells differentiated under normal (2% horse serum) condition. Under similar treatment, PPAR-gamma overexpressing cells resistant to insulin exhibited enhanced glucose uptake upto 60.7 +/- 4.08%, as compared to 23.8 +/- 5.1% observed in wild-type C2C12 skeletal muscle cells. These data demonstrate a direct involvement of PPAR-gamma in insulin sensitization of TZD action on skeletal muscle cells, and suggest that pharmacological overexpression of muscle PPAR-gamma gene in skeletal muscle might be a useful strategy for the treatment of insulin resistance.
Authors:
Navin K Verma; Jaskirat Singh; Chinmoy S Dey
Related Documents :
8971084 - Counterregulation in peripheral tissues: effect of systemic hypoglycemia on levels of s...
17106064 - Genetic model for the chronic activation of skeletal muscle amp-activated protein kinas...
24364194 - Surface emg of the masticatory muscles. (part 4): effects of occlusal splints and other...
3808314 - Myophosphorylase deficiency: the course of an unusual congenital myopathy.
2213604 - Proprioceptive guidance of human voluntary wrist movements studied using muscle vibration.
14586584 - Long-term spinal cord injury increases susceptibility to isometric contraction-induced ...
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2004-10-25
Journal Detail:
Title:  British journal of pharmacology     Volume:  143     ISSN:  0007-1188     ISO Abbreviation:  Br. J. Pharmacol.     Publication Date:  2004 Dec 
Date Detail:
Created Date:  2004-12-13     Completed Date:  2005-05-10     Revised Date:  2013-06-09    
Medline Journal Info:
Nlm Unique ID:  7502536     Medline TA:  Br J Pharmacol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1006-13     Citation Subset:  IM    
Affiliation:
Signal Transduction Research Laboratory, Department of Biotechnology, National Institute of Pharmaceutical Education and Research (NIPER), Sector 67, S.A.S. Nagar, Punjab 160 062, India.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Dose-Response Relationship, Drug
Gene Expression Regulation / physiology
Glucose / metabolism
Insulin / metabolism*,  pharmacology
Insulin Resistance / physiology
Mice
Muscle, Skeletal / cytology*,  drug effects,  metabolism*
PPAR gamma / biosynthesis*,  genetics
Chemical
Reg. No./Substance:
0/Insulin; 0/PPAR gamma; 50-99-7/Glucose
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Effects of grapefruit juice and orange juice components on P-glycoprotein- and MRP2-mediated drug ef...
Next Document:  Plant alkaloid tetrandrine downregulates IkappaBalpha kinases-IkappaBalpha-NF-kappaB signaling pathw...