Document Detail

PP2Cgamma-mediated S-phase accumulation induced by the proteasome-dependent degradation of p21(WAF1/CIP1).
MedLine Citation:
PMID:  17054950     Owner:  NLM     Status:  MEDLINE    
Serine/threonine phosphatases such as PP1, PP2A, and PP2B are well known to regulate the transition phase of the cell cycle. However, the function of PP2Cgamma in cell cycle progression is still unclear. In the present study, we report the characterization of PP2Cgamma in mammalian cells during the cell cycle. After release of synchronized cells from thymidine block, over-expression of PP2Cgamma led to accumulation in the S phase. The amount of endogenous p21(WAF1/CIP1) protein was markedly reduced by the expression of PP2Cgamma. The degradation of p21(WAF1/CIP1) induced by PP2Cgamma was mediated in a proteasome-dependent manner. In addition, the phosphatase activity of PP2Cgamma was capable of repressing the level of p21(WAF1/CIP1) protein. Phosphorylation of Rb was also reduced in cells expressing PP2Cgamma. Taken together, these results indicate that PP2Cgamma-induced S phase accumulation may be associated with proteasome-directed p21(WAF1/CIP1) degradation.
Eun-Jung Suh; Tae-Yoon Kim; Sang Hoon Kim
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-10-12
Journal Detail:
Title:  FEBS letters     Volume:  580     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  2006 Nov 
Date Detail:
Created Date:  2006-11-07     Completed Date:  2007-01-09     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  6100-4     Citation Subset:  IM    
Department of Biology, Kyung Hee University, Republic of Korea.
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MeSH Terms
Blotting, Western
Cell Cycle
Cell Line
Cyclin-Dependent Kinase Inhibitor p21 / analysis,  metabolism*
Flow Cytometry
Phosphoprotein Phosphatases / genetics,  physiology*
Phosphoric Monoester Hydrolases
Proteasome Endopeptidase Complex / metabolism
S Phase*
Reg. No./Substance:
0/CDKN1A protein, human; 0/Cyclin-Dependent Kinase Inhibitor p21; EC 3.1.3.-/Phosphoric Monoester Hydrolases; EC Phosphatases; EC phosphatase 2C; EC Endopeptidase Complex

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