Document Detail

PP2A is required for centromeric localization of Sgo1 and proper chromosome segregation.
MedLine Citation:
PMID:  16580887     Owner:  NLM     Status:  MEDLINE    
Loss of sister-chromatid cohesion triggers chromosome segregation in mitosis and occurs through two mechanisms in vertebrate cells: (1) phosphorylation and removal of cohesin from chromosome arms by mitotic kinases, including Plk1, during prophase, and (2) cleavage of centromeric cohesin by separase at the metaphase-anaphase transition. Bub1 and the MEI-S332/Shugoshin (Sgo1) family of proteins protect centromeric cohesin from mitotic kinases during prophase. We show that human Sgo1 binds to protein phosphatase 2A (PP2A). PP2A localizes to centromeres in a Bub1-dependent manner. The Sgo1-PP2A interaction is required for centromeric localization of Sgo1 and proper chromosome segregation in human cells. Depletion of Plk1 by RNA interference (RNAi) restores centromeric localization of Sgo1 and prevents chromosome missegregation in cells depleted of PP2A_Aalpha. Our findings suggest that Bub1 targets PP2A to centromeres, which in turn maintains Sgo1 at centromeres by counteracting Plk1-mediated chromosome removal of Sgo1.
Zhanyun Tang; Hongjun Shu; Wei Qi; Nadir A Mahmood; Marc C Mumby; Hongtao Yu
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2006-03-30
Journal Detail:
Title:  Developmental cell     Volume:  10     ISSN:  1534-5807     ISO Abbreviation:  Dev. Cell     Publication Date:  2006 May 
Date Detail:
Created Date:  2006-05-08     Completed Date:  2006-06-12     Revised Date:  2011-11-02    
Medline Journal Info:
Nlm Unique ID:  101120028     Medline TA:  Dev Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  575-85     Citation Subset:  IM    
Department of Pharmacology, The University of Texas Southwestern Medical Center, 6001 Forest Park Road, Dallas, Texas 75390, USA.
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MeSH Terms
Cell Cycle Proteins / metabolism*
Centromere / metabolism*
Chromosome Segregation*
Gene Expression
Hela Cells
Mutation / genetics
Phosphoprotein Phosphatases / metabolism*
Protein Binding
Protein Kinases / metabolism
Protein Phosphatase 2
Protein Structure, Tertiary
Protein Subunits
Protein Transport
Protein-Serine-Threonine Kinases / deficiency,  metabolism
Proto-Oncogene Proteins / deficiency,  metabolism
RNA Interference
Tumor Cells, Cultured
Grant Support
Reg. No./Substance:
0/Cell Cycle Proteins; 0/Protein Subunits; 0/Proto-Oncogene Proteins; 0/SGOL1 protein, human; EC 2.7.-/Protein Kinases; EC spindle checkpoint protein; EC Kinases; EC kinase 1; EC Phosphatases; EC Phosphatase 2
Comment In:
Dev Cell. 2006 May;10(5):544-7   [PMID:  16678770 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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