Document Detail


PO2 profiles near arterioles and tissue oxygen consumption in rat mesentery.
MedLine Citation:
PMID:  17483242     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A scanning phosphorescence quenching microscopy technique, designed to prevent accumulated O(2) consumption by the method, was applied to Po(2) measurements in mesenteric tissue. In an attempt to further increase the accuracy of the measurements, albumin-bound probe was topically applied to the tissue and an objective-mounted pressurized bag was used to reduce the oxygen transport bypass through the thin layer of fluid over the mesentery. Po(2) was measured at multiple sites perpendicular to the blood/wall interface in the vicinity of 84 mesenteric arterioles (7-39 microm in diameter) at distances of 5, 15, 30, 60, 120, and 180 microm in seven anesthetized Sprague-Dawley rats, thereby creating Po(2) profiles. Interstitial Po(2) above and immediately beside arterioles was found to agree with known intravascular values. No significant difference in Po(2) profiles was found between small and large arterioles, indicating a small longitudinal Po(2) gradient in the precapillary mesenteric microvasculature. In addition, the Po(2) profiles were used to calculate oxygen consumption in the mesenteric tissue (56-65 nl O(2) x cm(-3) x s(-1)). Correction of these values for contamination with ambient oxygen yielded an oxygen consumption rate of 60-68 nl O(2) x cm(-3) x s(-1), the maximal limit for consumption in the mesentery. The results were compared with measurements made by other workers in regard to the employed techniques.
Authors:
Aleksander S Golub; Matthew C Barker; Roland N Pittman
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2007-05-04
Journal Detail:
Title:  American journal of physiology. Heart and circulatory physiology     Volume:  293     ISSN:  0363-6135     ISO Abbreviation:  Am. J. Physiol. Heart Circ. Physiol.     Publication Date:  2007 Aug 
Date Detail:
Created Date:  2007-08-03     Completed Date:  2007-09-18     Revised Date:  2008-06-02    
Medline Journal Info:
Nlm Unique ID:  100901228     Medline TA:  Am J Physiol Heart Circ Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  H1097-106     Citation Subset:  IM    
Affiliation:
Department of Physiology, Medical College of Virginia Campus, Virginia Commonwealth University, 1101 E. Marshall St., PO Box 980551, Richmond, VA 23298-0551, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Arterioles / anatomy & histology,  metabolism
Artifacts*
Connective Tissue / metabolism*
Equipment Design
Female
Mesentery / blood supply*,  metabolism*
Microscopy, Fluorescence / instrumentation*
Models, Biological
Oxygen / blood,  metabolism*
Oxygen Consumption*
Partial Pressure
Rats
Rats, Sprague-Dawley
Reproducibility of Results
Grant Support
ID/Acronym/Agency:
HL18292/HL/NHLBI NIH HHS; HL79087/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
7782-44-7/Oxygen
Comments/Corrections
Erratum In:
Am J Physiol Heart Circ Physiol. 2008 May;294(5):H2410

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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