| PML3 interacts with TRF1 and is essential for ALT-associated PML bodies assembly in U2OS cells. | |
| | |
MedLine Citation:
|
PMID: 19900757 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Telomerase-negative cancer cells maintain their telomeres by a mechanism known as alternative lengthening of telomeres (ALT) and achieve unlimited replicative potential. A hallmark of ALT cells is the recruitment of telomeres to promyelocytic leukemia (PML) bodies and formation of ALT-associated PML bodies (APBs). Although the exact molecular mechanism of APBs assembly remains unclear, APBs assembly requires telomere and PML body-associated proteins, including TRF1 and PML. Here, we report that PML3, one of PML isoforms, is involved in APBs formation. As a new binding protein of TRF1 (telomeric repeat binding factor 1), PML3 directly interacts with TRF1 and recruits TRF1 to PML bodies in U2OS cells. More notably, depletion of PML3 by small interfering RNA does not affect PML bodies formation, but inhibits the recruitment of both TRF1 and TRF2 to APBs. Further study shows that the recruitment of TRF1 to APBs depends on its interaction with a specific PML3 isoform. Thus, the interaction of PML3 with TRF1 is isoform specific and likely to be essential for APBs assembly in U2OS cells. |
| | |
Authors:
|
Jian Yu; Jianping Lan; Chong Wang; Quan Wu; Yuanyuan Zhu; Xiaoyu Lai; Jie Sun; Changjiang Jin; He Huang |
Related Documents
:
|
8026567 - Nuclear matrix composition and in vitro cellular senescence. 12589817 - Immortalization with telomerase of the nestin-positive cells of the human pancreas. 8246307 - The effect of food chemicals on cell aging of human diploid cells in in vitro culture. 15976917 - An oncolytic adenovirus controlled by a modified telomerase promoter is attenuated in t... 15155267 - Na-k-2cl cotransporter inhibition impairs human lung cellular proliferation. 12583887 - A novel technique for inducing local inner ear damage. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-11-08 |
Journal Detail:
|
Title: Cancer letters Volume: 291 ISSN: 1872-7980 ISO Abbreviation: Cancer Lett. Publication Date: 2010 May |
Date Detail:
|
Created Date: 2010-04-05 Completed Date: 2010-04-23 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 7600053 Medline TA: Cancer Lett Country: Ireland |
Other Details:
|
Languages: eng Pagination: 177-86 Citation Subset: IM |
Affiliation:
|
The First Affiliated Hospital of Zhejiang University Medical School, Hangzhou 310003, China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Cell Cycle DNA, Complementary / genetics Fluorescent Antibody Technique Glutathione Transferase / metabolism Humans Kinetics Leukemia, Promyelocytic, Acute / genetics, metabolism, pathology Microsatellite Repeats Nuclear Proteins / genetics, metabolism* Palatine Tonsil / metabolism Protein Binding RNA, Small Interfering / genetics Recombinant Fusion Proteins / metabolism Recombinant Proteins / metabolism Reverse Transcriptase Polymerase Chain Reaction Telomeric Repeat Binding Protein 1 / genetics*, metabolism Transcription Factors / genetics, metabolism* Tumor Suppressor Proteins / genetics, metabolism* |
| Chemical | |
Reg. No./Substance:
|
0/DNA, Complementary; 0/Nuclear Proteins; 0/RNA, Small Interfering; 0/Recombinant Fusion Proteins; 0/Recombinant Proteins; 0/Telomeric Repeat Binding Protein 1; 0/Transcription Factors; 0/Tumor Suppressor Proteins; 143220-95-5/PML protein, human; EC 2.5.1.18/Glutathione Transferase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Decreased expression of microRNA-199b increases protein levels of SET (protein phosphatase 2A inhibi...
Next Document: Silibinin inhibits ethanol metabolism and ethanol-dependent cell proliferation in an in vitro model ...