Document Detail


PLXDC1 (TEM7) is identified in a genome-wide expression screen of glioblastoma endothelium.
MedLine Citation:
PMID:  17031559     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Glioblastomas are a highly aggressive brain tumor, with one of the highest rates of new blood vessel formation. In this study we used a combined experimental and bioinformatics strategy to determine which genes were highly expressed and specific for glioblastoma endothelial cells (GBM-ECs), compared to gene expression in normal tissue and endothelium. Starting from fresh glioblastomas, several rounds of negative and positive selection were used to isolate GBM-ECs and extract total RNA. Using Serial Analysis of Gene Expression (SAGE), 116,259 transcript tags (35,833 unique tags) were sequenced. From this expression analysis, we found 87 tags that were not expressed in normal brain. Further subtraction of normal endothelium, bone marrow, white blood cell and other normal tissue transcripts resulted in just three gene transcripts, ANAPC10, PLXDC1(TEM7), and CYP27B1, that are highly specific to GBM-ECs. Immunohistochemistry with an antibody for PLXDC1 showed protein expression in GBM microvasculature, but not in the normal brain endothelium tested. Our results suggest that this study succeeded in identifying GBM-EC specific genes. The entire gene expression profile for the GBM-ECs and other tissues used in this study are available at SAGE Genie (http://cgap.nci.nih.gov/SAGE). Functionally, the protein products of the three tags most specific to GBM-ECs have been implicated in processes critical to endothelial cell proliferation and differentiation, and are potential targets for anti-angiogenesis based therapy.
Authors:
Robert M Beaty; Jennifer B Edwards; Kathy Boon; I-Mei Siu; James E Conway; Gregory J Riggins
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2006-09-20
Journal Detail:
Title:  Journal of neuro-oncology     Volume:  81     ISSN:  0167-594X     ISO Abbreviation:  J. Neurooncol.     Publication Date:  2007 Feb 
Date Detail:
Created Date:  2007-01-22     Completed Date:  2007-04-05     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8309335     Medline TA:  J Neurooncol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  241-8     Citation Subset:  IM    
Affiliation:
Department of Neurosurgery, Johns Hopkins University Medical School, CRB II Rm. 257 , 1550 Orleans Street, Baltimore, MD, 21231, USA.
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MeSH Terms
Descriptor/Qualifier:
Brain Neoplasms / blood supply,  metabolism*
Computational Biology / methods*
Endothelial Cells / metabolism*
Endothelium, Vascular / metabolism
Expressed Sequence Tags
Gene Expression
Gene Expression Profiling
Gene Library
Glioblastoma / blood supply,  metabolism*
Humans
Immunohistochemistry
Neoplasm Proteins / biosynthesis*
Receptors, Cell Surface / biosynthesis*
Sequence Tagged Sites
Grant Support
ID/Acronym/Agency:
R01 NS052507/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Neoplasm Proteins; 0/PLXDC1 protein, human; 0/Receptors, Cell Surface

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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