Document Detail

PLAC4 and beta-HCG mRNA levels are not altered in the maternal circulation of pregnancies with trisomy 21.
MedLine Citation:
PMID:  19097032     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: Beta-human chorionic gonadotropin (HCG) and pregnancy-associated plasma protein (PAPP-A) are placentally produced proteins whose levels are altered in pregnancies with trisomy 21. PLAC4 is located on chromosome 21 and its expression is restricted to the placenta. Here we investigated whether the levels of beta-HCG-, PAPP-A- and PLAC4 mRNA could be able to discriminate pregnancies whose fetus is affected by trisomy 21. METHOD: Hundred and forty-three blood samples from normal pregnancies and eight samples from trisomic pregnancies were collected. Total RNA was extracted from whole maternal blood, reverse-transcribed and the three mRNAs were quantified by real-time quantitative PCR. Hundred and nine controls were also tested for the serum levels of PAPP-A and HCG proteins. RESULTS: Beta-HCG and PLAC4 mRNAs were detected in all samples, in higher amounts than in plasma, whereas the detection rate for PAPP-A mRNA was below 10%. The levels of beta-HCG mRNA significantly correlated with the circulatory concentrations of the HCG protein. However, neither beta-HCG- nor PLAC4 mRNAs show a significant difference between cases and controls. CONCLUSION: Maternal blood levels of beta-HCG-, PLAC4- and PAPP-A mRNAs are not useful markers for the screening of pregnancies with trisomy 21 as their concentrations are either not significantly altered (beta-HCG and PLAC4) or too low to be detected (PAPP-A).
Irina Banzola; Corinne Rusterholz; Letizia Zannoni; Nicola Rizzo; Xiao Yan Zhong; Elisabetta Caramelli; Wolfgang Holzgreve; Antonio Farina; Sinuhe Hahn
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Prenatal diagnosis     Volume:  28     ISSN:  0197-3851     ISO Abbreviation:  Prenat. Diagn.     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2008-12-24     Completed Date:  2009-03-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8106540     Medline TA:  Prenat Diagn     Country:  England    
Other Details:
Languages:  eng     Pagination:  1262-7     Citation Subset:  IM    
Laboratory for Prenatal Medicine and Gyn. Oncology, Department of Biomedicine and University Hospital, Basel, Switzerland.
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MeSH Terms
Case-Control Studies
Chorionic Gonadotropin, beta Subunit, Human / biosynthesis*,  blood,  genetics
Down Syndrome / blood,  genetics*
Pregnancy-Associated Plasma Protein-A / biosynthesis*,  genetics
Prenatal Diagnosis / methods*
RNA, Messenger / genetics,  metabolism
Reverse Transcriptase Polymerase Chain Reaction
Reg. No./Substance:
0/Chorionic Gonadotropin, beta Subunit, Human; 0/RNA, Messenger; EC 3.4.24.-/Pregnancy-Associated Plasma Protein-A

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