Document Detail


PKH26 can transfer to host cells in vitro and vivo.
MedLine Citation:
PMID:  22913652     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The fluorescent dye, PKH26, which mainly binds to the cell membrane, has been used as the cell tracer to locate the transplanted cells in host for a long time. However, there was no detailed report that whether the PKH26 dye was specific to the transplanted cells. Therefore, the aim of this article is to explore the effect of cells debris as the cracking cells from the PKH26-labeled adipose-derived stem cells (ADSCs) on the cells in vitro and the host in vivo. After we tested the proliferation and toxicity of PKH26 to the ADSCs by the Cell Count-8 kit and alamar blue assay, we constructed 2 models, coculturing lots of PKH26-labeled cell debris with the unlabeled ADSCs in vitro and injecting via the tail vein in rat, to evaluate the specificity of the PKH26 dye. The result indicated that the PKH26 didn't inhibit the proliferation and had no toxicity to the ADSCs compared with the unlabeled ADSCs, but the cell debris cracking from PKH26-labeled transplanted cells can cause the unlabeled cells to emit red fluorescence in vitro and also lead the tissues displaying red fluorescence in vivo. We can conclude that the PKH26 dye, used as a cell tracer for a long time, was not an ideal cell tracer.
Authors:
Peng Li; Run Zhang; Haitao Sun; Lei Chen; Fang Liu; Chen Yao; Mouxuan Du; Xiaodan Jiang
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-10-19
Journal Detail:
Title:  Stem cells and development     Volume:  22     ISSN:  1557-8534     ISO Abbreviation:  Stem Cells Dev.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-01-11     Completed Date:  2013-07-02     Revised Date:  2013-07-12    
Medline Journal Info:
Nlm Unique ID:  101197107     Medline TA:  Stem Cells Dev     Country:  United States    
Other Details:
Languages:  eng     Pagination:  340-4     Citation Subset:  IM    
Affiliation:
Department of Neurosurgery, Southern Medical University, Zhujiang Hospital, Guangzhou, China.
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MeSH Terms
Descriptor/Qualifier:
Adipose Tissue / cytology
Animals
Animals, Newborn
Cell Count
Cell Proliferation
Cell Shape
Cell Transplantation / methods
Coculture Techniques / methods
Fluorescence
Fluorescent Dyes / metabolism*,  toxicity
Kidney / metabolism
Liver / metabolism
Models, Animal
Organic Chemicals / metabolism*,  toxicity
Oxazines / metabolism
Rats
Rats, Wistar
Staining and Labeling / methods*
Stem Cells / drug effects,  metabolism*
Toxicity Tests
Xanthenes / metabolism
Chemical
Reg. No./Substance:
0/Fluorescent Dyes; 0/Organic Chemicals; 0/Oxazines; 0/PKH 26; 0/Xanthenes; 550-82-3/resazurin
Comments/Corrections

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