| PKCγ participates in food entrainment by regulating BMAL1. | |
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MedLine Citation:
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PMID: 23185022 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Temporally restricted feeding (RF) can phase reset the circadian clocks in numerous tissues in mammals, contributing to altered timing of behavioral and physiological rhythms. However, little is known regarding the underlying molecular mechanism. Here we demonstrate a role for the gamma isotype of protein kinase C (PKCγ) in food-mediated entrainment of behavior and the molecular clock. We found that daytime RF reduced late-night activity in wild-type mice but not mice homozygous for a null mutation of PKCγ (PKCγ(-/-)). Molecular analysis revealed that PKCγ exhibited RF-induced changes in activation patterns in the cerebral cortex and that RF failed to substantially phase shift the oscillation of clock gene transcripts in the absence of PKCγ. PKCγ exerts effects on the clock, at least in part, by stabilizing the core clock component brain and muscle aryl hydrocarbon receptor nuclear translocator like 1 (BMAL1) and reducing its ubiquitylation in a deubiquitination-dependent manner. Taken together, these results suggest that PKCγ plays a role in food entrainment by regulating BMAL1 stability. |
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Authors:
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Luoying Zhang; Diya Abraham; Shu-Ting Lin; Henrik Oster; Gregor Eichele; Ying-Hui Fu; Louis J Ptáček |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2012-11-26 |
Journal Detail:
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Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 109 ISSN: 1091-6490 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 2012 Dec |
Date Detail:
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Created Date: 2012-12-13 Completed Date: 2013-02-28 Revised Date: 2013-06-12 |
Medline Journal Info:
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Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: United States |
Other Details:
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Languages: eng Pagination: 20679-84 Citation Subset: IM |
Affiliation:
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Department of Neurology, University of California, San Francisco, CA 94158, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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ARNTL Transcription Factors
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genetics,
physiology* Animals Cerebral Cortex / physiology Circadian Rhythm / genetics, physiology* Eating / genetics, physiology Feeding Behavior / physiology* Male Mice Mice, 129 Strain Mice, Inbred C57BL Mice, Knockout Models, Neurological Mutation Photoperiod Protein Kinase C / deficiency, genetics, physiology* Protein Stability Signal Transduction Ubiquitination |
| Grant Support | |
ID/Acronym/Agency:
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GM079180/GM/NIGMS NIH HHS; HL059596/HL/NHLBI NIH HHS; //Howard Hughes Medical Institute |
| Chemical | |
Reg. No./Substance:
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0/ARNTL Transcription Factors; 0/Arntl protein, mouse; EC 2.7.1.-/protein kinase C gamma; EC 2.7.11.13/Protein Kinase C |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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