| PKC inhibition is involved in trichosanthin-induced apoptosis in human chronic myeloid leukemia cell line K562. | |
| | |
MedLine Citation:
|
PMID: 16949756 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Trichosanthin (TCS), a type I ribosome-inactivating protein, induces cell death in various cell types including several tumor cell lines. However, the mechanism remains largely uncharacterized. In this study, we investigated the possible mechanism underlying its cytotoxicity by using human chronic myeloid leukemia cell line K562. We found that TCS induced apoptosis in K562 cells in a time- and concentration-dependent manner and can be blocked by caspase-3 inhibitors. Interestingly, TCS treatment induced a transient elevation in intracellular calcium concentration and a slow increase in reactive oxygen species production, while calcium chelators and antioxidants had no obvious effect on TCS-induced apoptosis, suggesting that calcium changes and reactive oxygen species may not be involved in TCS-mediated apoptosis in K562 cells. Instead we found that TCS partly inhibited PKC activity. Indeed, the PKC activator, PMA, inhibited while the PKC inhibitor, calphostin c, enhanced TCS-induced apoptosis. These PKC modulators had similar effects on TCS-induced cleavage of caspase-3, and caspase-3 inhibitors prevented calphostin c-enhanced apoptosis induced by TCS. In summary, we conclude that TCS induces apoptosis in K562 cells partly via PKC inhibition and caspase-3 activation. |
| | |
Authors:
|
Jie Li; Xuechun Xia; Huiling Nie; Mark A Smith; Xiongwei Zhu |
Related Documents
:
|
15289336 - Sensitization to the lysosomal cell death pathway upon immortalization and transformation. 9535846 - Stat1 is inactivated by a caspase. 10495426 - Involvement of cyclin d1-cdk5 overexpression and mcm3 cleavage in bax-associated sponta... 7615196 - Poly(adenosine diphosphate ribose) polymerase inhibition prevents necrosis induced by h... 15265666 - Prolactin stimulates maturation and function of rat thymic dendritic cells. 17234676 - Endometriosis: an inflammatory disease with a th2 immune response component. |
Publication Detail:
|
Type: Journal Article Date: 2006-07-26 |
Journal Detail:
|
Title: Biochimica et biophysica acta Volume: 1770 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 2007 Jan |
Date Detail:
|
Created Date: 2006-12-20 Completed Date: 2007-02-13 Revised Date: 2007-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 63-70 Citation Subset: IM |
Affiliation:
|
Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Science, Graduate School of the Chinese Academy of Sciences, 320 Yue-Yang Road, Shanghai 200031, China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Apoptosis
/
drug effects* Calcium / metabolism Calcium Signaling Caspase 3 / metabolism Flow Cytometry Humans Microscopy, Confocal Naphthalenes / pharmacology* Protein Kinase C / antagonists & inhibitors* Protein Kinase Inhibitors / pharmacology* Reactive Oxygen Species Trichosanthin / pharmacology* |
| Chemical | |
Reg. No./Substance:
|
0/Naphthalenes; 0/Protein Kinase Inhibitors; 0/Reactive Oxygen Species; 121263-19-2/calphostin C; 60318-52-7/Trichosanthin; 7440-70-2/Calcium; EC 2.7.11.13/Protein Kinase C; EC 3.4.22.-/Caspase 3 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Optimizing phase contrast in transmission electron microscopy with an electrostatic (Boersch) phase ...
Next Document: Specific peptide patterns of follicular fluids at different growth stages analyzed by matrix-assiste...