Document Detail

PKCθ expression in the amygdala regulates insulin signaling, food intake and body weight.
MedLine Citation:
PMID:  23712979     Owner:  NLM     Status:  In-Data-Review    
OBJECTIVE: To investigate the signaling mechanisms that might underlie the loss of anorectic response to insulin injections into the central nucleus of the amygdala (CeA) within 3 days of feeding a high fat diet.
DESIGN AND METHODS: Protein samples from amygdala and hypothalamus of rats fed high or low fat diets were subjected to a phosphorylation screening assay. The effects of dietary fat intake on the expression and activation of protein kinase C theta (PKCθ) in brain regions was studied. Finally, lentiviral vectors were used to overexpress rat PKCθ unilaterally or bilaterally into the CeA of rats and the effects on food intake, body weight and insulin stimulation of Akt phosphorylation were studied.
RESULTS: The level of pMARCKS (Myristoylated alanine-rich C-kinase substrate), a major substrate of PKCθ, was increased 116% in amygdala of high fat diet fed rats but reduced in the hypothalamus. High fat diets increased the level of PKCθ in a region specific manner in the brain and this PKCθ was activated by membrane association. Overexpressing rat PKCθ either unilaterally or bilaterally into the CeA inhibited insulin stimulation of Akt signaling and blocked the anorectic response to insulin injected into the amygdala. Bilaterally injected PKCθ rats gained more weight and body fat and had increased food intake when fed a high fat diet compared to the control rats that received a lentiviral-Green Fluorescent Protein construct.
CONCLUSION: The data suggest that insulin may have a physiological role within the amygdala to regulate energy balance.
Miejung Park-York; Stéphane Boghossian; Hyoungil Oh; David A York
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Obesity (Silver Spring, Md.)     Volume:  21     ISSN:  1930-739X     ISO Abbreviation:  Obesity (Silver Spring)     Publication Date:  2013 Apr 
Date Detail:
Created Date:  2013-05-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101264860     Medline TA:  Obesity (Silver Spring)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  755-64     Citation Subset:  IM    
Copyright Information:
Copyright © 2013 The Obesity Society.
Department of Biology, Utah State University, Logan, Utah, USA.
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