Document Detail

PKC activity in rat C6 glioma cells: changes associated with cell cycle and simvastatin treatment.
MedLine Citation:
PMID:  8179595     Owner:  NLM     Status:  MEDLINE    
The parallel effects of simvastatin on cell cycle and PKC activity in rat C6 glioma cells were investigated. Simvastatin, 2.5 microM, for 24 h resulted in cell growth arrest in early G1 phase of the cell cycle and in a significant increase of total PKC activity (283 +/- 42 vs 470 +/- 61 pmoles/min/mg protein p = 0.002 for control cells and simvastatin-treated cells, respectively). The effect of simvastatin was fully prevented by mevalonate. A time dependent increase of PKC activity was observed in control exponentially free-growing C6 cells approaching confluency: a highly significant negative correlation (r = -0.91 p < 0.0001) between PKC activity and growth rate was calculated. PKC activity was high in cells arrested in G0 by serum starvation (0.4%). Following addition of complete medium (17.5% serum) the PKC activity progressively decreased and reached a minimum when cells traversed the G2/M phase, as determined by DNA analysis distribution. PKC activity dropped 30% in simvastatin-arrested early G1 cells; 44% in hydroxyurea-arrested cells at the G1/S boundary; and 73% in Colcemid mitosis-blocked cells. The results show that C6 glioma cell PKC activity is maximal in a G0 quiescent state and varies at different points of the cell cycle.
M R Soma; R Baetta; S Bergamaschi; M R De Renzis; C Davegna; F Battaini; R Fumagalli; S Govoni
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  200     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1994 Apr 
Date Detail:
Created Date:  1994-06-03     Completed Date:  1994-06-03     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1143-9     Citation Subset:  IM    
Institute of Pharmacological Sciences, University of Milan, Italy.
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MeSH Terms
Astrocytoma / enzymology,  pathology
Cell Cycle / drug effects
Demecolcine / pharmacology
Glioma / enzymology*,  pathology
Hydroxymethylglutaryl-CoA Reductase Inhibitors
Hydroxyurea / pharmacology
Lovastatin / analogs & derivatives*,  antagonists & inhibitors,  pharmacology
Mevalonic Acid / pharmacology
Protein Kinase C / metabolism*
Tumor Cells, Cultured / drug effects,  enzymology,  pathology
Reg. No./Substance:
0/Hydroxymethylglutaryl-CoA Reductase Inhibitors; 127-07-1/Hydroxyurea; 150-97-0/Mevalonic Acid; 477-30-5/Demecolcine; 75330-75-5/Lovastatin; 79902-63-9/Simvastatin; EC Kinase C

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