| PKCθ Expression in the Amygdala Regulates Insulin Signaling, Food Intake and Body Weight. | |
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MedLine Citation:
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PMID: 22836691 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Insulin injections into the central nucleus of the amygdala (CeA) reduce food intake, but this response is lost within 3 days of feeding a high fat diet (HFD). To investigate the signaling mechanisms that might underlie this loss of response to insulin, we subjected protein samples from amygdala and hypothalamus to a phosphorylation screening assay. The level of myristoylated alanine-rich C-kinase substrate, a major substrate of protein kinase Cθ (PKCθ), was increased 116% in amygdala of HFD-fed rats, but reduced in the hypothalamus. HFDs increased the level of PKCθ in a region specific manner in the brain and this PKCθ was activated by membrane association. Overexpressing rat PKCθ either unilaterally or bilaterally into the CeA of rats using a lentiviral construct inhibited insulin stimulation of Akt signaling and blocked the anorectic response to insulin injected into the amygdala. Bilaterally injected PKCθ rats gained more weight and body fat and had increased food intake when fed a HFD compared with the control rats that received a lentiviral-green fluorescent protein construct. The data suggest that insulin may have a physiological role within the amygdala to regulate energy balance. |
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Authors:
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Miejung Park-York; Stéphane Boghossian; Hyoungil Oh; David A York |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-6-22 |
Journal Detail:
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Title: Obesity (Silver Spring, Md.) Volume: - ISSN: 1930-7381 ISO Abbreviation: - Publication Date: 2012 Jun |
Date Detail:
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Created Date: 2012-7-27 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101264860 Medline TA: Obesity (Silver Spring) Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Department of Biology, Utah State University, Logan, Utah, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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