Document Detail


A PIP-family protein is required for biosynthesis of tobacco alkaloids.
MedLine Citation:
PMID:  19002761     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Plants in the Nicotiana genus produce nicotine and related pyridine alkaloids as a part of their chemical defense against insect herbivores. These alkaloids are formed by condensation of a derivative of nicotinic acid, but the enzyme(s) involved in the final condensation step remains elusive. In Nicotiana tabacum, an orphan reductase A622 and its close homolog A622L are coordinately expressed in the root, upregulated by methyl jasmonate treatment, and controlled by the NIC regulatory loci specific to the biosynthesis of tobacco alkaloids. Conditional suppression of A622 and A622L by RNA interference inhibited cell growth, severely decreased the formation of all tobacco alkaloids, and concomitantly induced an accumulation of nicotinic acid beta-N-glucoside, a probable detoxification metabolite of nicotinic acid, in both hairy roots and methyl jasmonate-elicited cultured cells of tobacco. N-methylpyrrolinium cation, a precursor of the pyrrolidine moiety of nicotine, also accumulated in the A622(L)-knockdown hairy roots. We propose that the tobacco A622-like reductases of the PIP family are involved in either the formation of a nicotinic acid-derived precursor or the final condensation reaction of tobacco alkaloids.
Authors:
Masataka Kajikawa; Nobuhiro Hirai; Takashi Hashimoto
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-11-12
Journal Detail:
Title:  Plant molecular biology     Volume:  69     ISSN:  0167-4412     ISO Abbreviation:  Plant Mol. Biol.     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2009-01-21     Completed Date:  2009-03-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9106343     Medline TA:  Plant Mol Biol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  287-98     Citation Subset:  IM    
Affiliation:
Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama-cho, Ikoma, Nara, 630-0192, Japan.
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MeSH Terms
Descriptor/Qualifier:
Alkaloids / biosynthesis*,  chemistry,  genetics*
Immunoblotting
Molecular Structure
Nicotine / chemistry,  metabolism
Nicotinic Acids / metabolism
Phylogeny
Plant Proteins / classification,  genetics,  physiology*
Plant Roots / genetics,  growth & development,  metabolism
Plants, Genetically Modified / genetics,  growth & development,  metabolism
Pyrroles / chemistry,  metabolism
Reverse Transcriptase Polymerase Chain Reaction
Tobacco / genetics,  growth & development,  metabolism*
Chemical
Reg. No./Substance:
0/Alkaloids; 0/Nicotinic Acids; 0/Plant Proteins; 0/Pyrroles; 16032-02-3/1-methyl-delta(1)-pyrrolinium; 54-11-5/Nicotine

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