Document Detail


PIASy interacts with p73alpha and regulates cell cycle in HEK293 cells.
MedLine Citation:
PMID:  20471636     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
p73, a recently described member of the p53 family of transcription factors, undergoes a number of posttranslational modifications, inducing cell cycle arrest and apoptosis. In the present study, we demonstrate that PIASy, a member of the PIAS SUMO-ligase family, interacts with p73alpha, and that PIASy-mediated sumoylation promotes proteasomal degradation of p73alpha. PIASy overexpression inhibits p73alpha-mediated transcription of p21, with a reduction of cells in G1 and cell cycle reentry. These results suggest that PIASy plays an important role in regulation of p73alpha transcriptional activity and is also a regulator of the cell cycle machinery.
Authors:
Chao Zhang; Xia Yuan; Ling Yue; Jin Fu; Lan Luo; Zhimin Yin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-04-18
Journal Detail:
Title:  Cellular immunology     Volume:  263     ISSN:  1090-2163     ISO Abbreviation:  Cell. Immunol.     Publication Date:  2010  
Date Detail:
Created Date:  2010-06-10     Completed Date:  2010-07-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  1246405     Medline TA:  Cell Immunol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  235-40     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2010 Elsevier Inc. All rights reserved.
Affiliation:
Jiangsu Province Key Laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210046, PR China.
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MeSH Terms
Descriptor/Qualifier:
Cell Cycle / physiology*
Cell Line
DNA-Binding Proteins / metabolism*
Gene Expression
Humans
Immunoblotting
Nuclear Proteins / metabolism*
Protein Binding
Protein Inhibitors of Activated STAT / metabolism*
Protein Processing, Post-Translational*
Reverse Transcriptase Polymerase Chain Reaction
Small Ubiquitin-Related Modifier Proteins / metabolism
Tumor Suppressor Proteins / metabolism*
Chemical
Reg. No./Substance:
0/DNA-Binding Proteins; 0/Nuclear Proteins; 0/Protein Inhibitors of Activated STAT; 0/Small Ubiquitin-Related Modifier Proteins; 0/Tumor Suppressor Proteins; 0/tumor suppressor protein p73

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