| PI3Kγ activation is required for LPS-induced reactive oxygen species generation in respiratory epithelial cells. | |
| | |
MedLine Citation:
|
PMID: 22825625 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
OBJECTIVE: In this study, we investigated the molecular basis of reactive oxygen species (ROS) generation induced by lipopolysaccharide (LPS) in A549 cells-an alveolar epithelial cell line. EXPERIMENTAL DESIGN: A549 cells or normal human bronchial epithelial (NHBE) cells were stimulated with LPS. ROS generation was measured in A549 cells or NHBE cells pre-treated with a selective inhibitor of phosphatidylinositol 3-kinase γ (PI3Kγ), AS 605240, PI3Kγ siRNA, or a ROS scavenger, pyridoxamine (PM). RESULTS: Treatment of A549 cells or NHBE cells with LPS caused a significant increase in intracellular ROS generation. Pretreatment with the PI3Kγ inhibitor, AS 605240 decreased the LPS-induced increase of ROS generation, phosphorylation of Akt, and production of phosphatidyl 3,4,5-trisphosphate in A549 cells. In addition, interference with siRNA for PI3Kγ significantly reduced LPS-induced ROS generation in A549 cells. Treatment of A549 cells with LPS or hydrogen peroxide increased the nuclear factor-κB (NF-κB) in the nucleus, accompanying an increase in phosphorylation of inhibitory κB-α, degradation of the protein, and reduction of cytosolic NF-κB. Pretreatment with AS 605240 reduced these LPS-induced changes. In addition, pretreatment with PM or N-acetyl cysteine resulted in inhibition of nuclear NF-κB activation. CONCLUSION: These results suggest that PI3Kγ plays a key role in LPS-induced ROS generation in alveolar epithelial cells, thereby activating NF-κB. |
| | |
Authors:
|
Hee Jung Kim; So Ri Kim; Jin Kyeong Park; Dong Im Kim; Jae Seok Jeong; Yong Chul Lee |
Related Documents
:
|
22828925 - Down-regulation of mir-124/-214 in cutaneous squamous cell carcinoma mediates abnormal ... 21153115 - Effects of luteinizing hormone and prostaglandin f(2α) on gap junctional intercellular... 9665185 - Large cell lymphoma complicating persistent polyclonal b cell lymphocytosis. 2860765 - Cytologic demonstration of entamoeba histolytica using immunoperoxidase techniques. rep... 18006285 - Localized quorum sensing in vibrio fischeri. 21635945 - Lactate is an ideal non-invasive marker for evaluating temporal alterations in cell str... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-7-24 |
Journal Detail:
|
Title: Inflammation research : official journal of the European Histamine Research Society ... [et al.] Volume: - ISSN: 1420-908X ISO Abbreviation: - Publication Date: 2012 Jul |
Date Detail:
|
Created Date: 2012-7-24 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9508160 Medline TA: Inflamm Res Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Department of Internal Medicine, Research Center for Pulmonary Disorders, Chonbuk National University Medical School, San 2-20 Geumam-dong, Deokjin-gu, Jeonbuk, Jeonju, 561-180, South Korea. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Differentiation of bone marrow-derived mesenchymal stem cells into chondrocytes using chondrocyte ex...
Next Document: Postconditioning with vagal stimulation attenuates local and systemic inflammatory responses to myoc...