Document Detail


PHO1 expression in guard cells mediates the stomatal response to abscisic acid in Arabidopsis.
MedLine Citation:
PMID:  22612335     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Stomatal opening and closing are driven by ion fluxes that cause changes in guard cell turgor and volume. This process is, in turn, regulated by environmental and hormonal signals, including light and the phytohormone abscisic acid (ABA). Here, we present genetic evidence that expression of PHO1 in guard cells of Arabidopsis thaliana is required for full stomatal responses to ABA. PHO1 is involved in the export of phosphate into the root xylem vessels and, as a result, the pho1 mutant is characterized by low shoot phosphate levels. In leaves, PHO1 was found expressed in guard cells and up-regulated following treatment with ABA. The pho1 mutant was unaffected in ROS production following ABA treatment, and in stomatal movements in response to light cues, high extracellular calcium, auxin, and fusicoccin. However, stomatal movements in response to ABA treatment were severely impaired, both in terms of induction of closure and inhibition of opening. Micro-grafting a pho1 shoot scion onto wild-type rootstock resulted in plants with normal shoot growth and Pi content, but failed to restore normal stomatal response to ABA treatment. PHO1 knockdown using RNAi specifically in guard cells of wild-type plants caused a reduced stomatal response to ABA. In agreement, specific expression of PHO1 in guard cells of pho1 plants complemented the mutant guard cell phenotype and re-established ABA sensitivity, although full functional complementation was dependent on shoot Pi sufficiency. Together, these data reveal an important role for phosphate and PHO1 action in the stomatal response to ABA.
Authors:
Céline Zimmerli; Cécile Ribot; Alain Vavasseur; Hubert Bauer; Rainer Hedrich; Yves Poirier
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-5-21
Journal Detail:
Title:  The Plant journal : for cell and molecular biology     Volume:  -     ISSN:  1365-313X     ISO Abbreviation:  -     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-5-22     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9207397     Medline TA:  Plant J     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 The Authors. The Plant Journal © 2012 Blackwell Publishing Ltd.
Affiliation:
Département de Biologie Moléculaire Végétale, Biophore, Université de Lausanne, CH-1015 Lausanne, Switzerland CEA/CNRS, Laboratoire de Biologie du Développement des Plantes, UMR 7265, Université Aix-Marseille, St Paul Lez-Durance F-13108, France. Institute for Molecular Plant Physiology and Biophysics, University Würzburg, Julius-von-Sachs Platz 2, D-97082 Würzburg, Germany.
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