Document Detail

PGE receptor characteristics on porcine luteal cells during the estrous cycle and early pregnancy.
MedLine Citation:
PMID:  10367293     Owner:  NLM     Status:  MEDLINE    
This study examined the affinities and concentrations of prostaglandin E (PGE) receptors on porcine luteal cells during the estrous cycle and early pregnancy. Corpora lutea (CL) were obtained from nonpregnant gilts at days 9 (n = 4), 12 (n = 3), and 14 (n = 6); three gilts possessed red, vascular CL and three gilts had white nonvascular CL) of the estrous cycle, and days 9 (n = 4), 12 (n = 3), 14 (n = 5), and 30 (n = 5) of pregnancy. The CL were dissociated enzymatically to disperse single cells and the red blood cells were removed by elutriation. The luteal cells were assayed for specific PGE binding by displacement analysis with use of [3H] PGE2 and varying concentrations of unlabeled PGE2. The specific binding of [3H] PGE2 to luteal cells decreased (p < 0.05) from days 9 to 14 of the estrous cycle, but only decreased (p < 0.05) from days 9 to 12 of pregnancy. Specific binding was higher (p < 0.05) on day 14 of pregnancy than the comparable stage of the estrous cycle. The affinities of PGE receptors decreased (p < 0.05) only on the luteal cells dissociated from red, vascular CL of day 14 nonpregnant gilts compared with those of other days of the estrous cycle and pregnancy. The number of PGE receptors on porcine luteal cells was similar (p > 0.05) in pregnant and nonpregnant gilts, but decreased (p < 0.05) on days 12-14 postestrus. During early pregnancy, it was evident that high affinity PGE receptors are sustained on porcine luteal cells; however, the role of the PGE receptors in maternal recognition of pregnancy remains speculative.
S M Feng; G W Almond
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Prostaglandins & other lipid mediators     Volume:  57     ISSN:  1098-8823     ISO Abbreviation:  Prostaglandins Other Lipid Mediat.     Publication Date:  1999 Jan 
Date Detail:
Created Date:  1999-07-15     Completed Date:  1999-07-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9808648     Medline TA:  Prostaglandins Other Lipid Mediat     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  13-22     Citation Subset:  IM    
Department of Food Animal and Equine Medicine, College of Veterinary Medicine, North Carolina State University, Raleigh 27606, USA.
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MeSH Terms
Corpus Luteum / metabolism*
Dinoprostone / metabolism
Pregnancy, Animal / metabolism*
Progesterone / blood
Radioligand Assay
Receptors, Prostaglandin E / metabolism*
Reg. No./Substance:
0/Receptors, Prostaglandin E; 363-24-6/Dinoprostone; 57-83-0/Progesterone

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