| PGE receptor characteristics on porcine luteal cells during the estrous cycle and early pregnancy. | |
| | |
MedLine Citation:
|
PMID: 10367293 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
This study examined the affinities and concentrations of prostaglandin E (PGE) receptors on porcine luteal cells during the estrous cycle and early pregnancy. Corpora lutea (CL) were obtained from nonpregnant gilts at days 9 (n = 4), 12 (n = 3), and 14 (n = 6); three gilts possessed red, vascular CL and three gilts had white nonvascular CL) of the estrous cycle, and days 9 (n = 4), 12 (n = 3), 14 (n = 5), and 30 (n = 5) of pregnancy. The CL were dissociated enzymatically to disperse single cells and the red blood cells were removed by elutriation. The luteal cells were assayed for specific PGE binding by displacement analysis with use of [3H] PGE2 and varying concentrations of unlabeled PGE2. The specific binding of [3H] PGE2 to luteal cells decreased (p < 0.05) from days 9 to 14 of the estrous cycle, but only decreased (p < 0.05) from days 9 to 12 of pregnancy. Specific binding was higher (p < 0.05) on day 14 of pregnancy than the comparable stage of the estrous cycle. The affinities of PGE receptors decreased (p < 0.05) only on the luteal cells dissociated from red, vascular CL of day 14 nonpregnant gilts compared with those of other days of the estrous cycle and pregnancy. The number of PGE receptors on porcine luteal cells was similar (p > 0.05) in pregnant and nonpregnant gilts, but decreased (p < 0.05) on days 12-14 postestrus. During early pregnancy, it was evident that high affinity PGE receptors are sustained on porcine luteal cells; however, the role of the PGE receptors in maternal recognition of pregnancy remains speculative. |
| | |
Authors:
|
S M Feng; G W Almond |
Related Documents
:
|
15189823 - Secreted and placental membrane forms of folate-binding protein occur sequentially duri... 9622293 - Distribution and fine structure of macrophages in the human ovary during the menstrual ... 12165283 - Treatment of deep venous thrombosis with low-molecular-weight heparin during pregnancy. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Prostaglandins & other lipid mediators Volume: 57 ISSN: 1098-8823 ISO Abbreviation: Prostaglandins Other Lipid Mediat. Publication Date: 1999 Jan |
Date Detail:
|
Created Date: 1999-07-15 Completed Date: 1999-07-15 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 9808648 Medline TA: Prostaglandins Other Lipid Mediat Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 13-22 Citation Subset: IM |
Affiliation:
|
Department of Food Animal and Equine Medicine, College of Veterinary Medicine, North Carolina State University, Raleigh 27606, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Corpus Luteum / metabolism* Dinoprostone / metabolism Estrus* Female Pregnancy Pregnancy, Animal / metabolism* Progesterone / blood Radioimmunoassay Radioligand Assay Receptors, Prostaglandin E / metabolism* Swine |
| Chemical | |
Reg. No./Substance:
|
0/Receptors, Prostaglandin E; 363-24-6/Dinoprostone; 57-83-0/Progesterone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Effects of angiogenic growth factors on endothelium-derived prostacyclin production by ovine uterine...
Next Document: Opiate, cannabinoid, and eicosanoid signaling converges on common intracellular pathways nitric oxid...