| PGC-1α overexpression results in increased hepatic fatty acid oxidation with reduced triacylglycerol accumulation and secretion. | |
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MedLine Citation:
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PMID: 22899824 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Studies have shown that decreased mitochondrial content and function are associated with hepatic steatosis. We examined whether peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α) overexpression and a subsequent increase in mitochondrial content and function in rat primary hepatocytes (in vitro) and Sprague-Dawley rats (in vivo) would comprehensively alter mitochondrial lipid metabolism, including complete (CO(2)) and incomplete (acid-soluble metabolites) fatty acid oxidation (FAO), tricarboxylic acid cycle flux, and triacylglycerol (TAG) storage and export. PGC-1α overexpression in primary hepatocytes produced an increase in markers of mitochondrial content and function (citrate synthase, mitochondrial DNA, and electron transport system complex proteins) and an increase in FAO, which was accompanied by reduced TAG storage and TAG secretion compared with control. Also, the PGC-1α-overexpressing hepatocytes were protected from excess TAG accumulation following overnight lipid treatment. PGC-1α overexpression in hepatocytes lowered expression of genes critical to VLDL assembly and secretion (apolipoprotein B and microsomal triglyceride transfer protein). Adenoviral transduction of rats with PGC-1α resulted in a liver-specific increase in PGC-1α expression and produced an in vivo liver phenotype of increased FAO via increased mitochondrial function that also resulted in reduced hepatic TAG storage and decreased plasma TAG levels. In conclusion, overexpression of hepatic PGC-1α and subsequent increases in FAO through elevated mitochondrial content and/or function result in reduced TAG storage and secretion in the in vitro and in vivo milieu. |
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Authors:
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E Matthew Morris; Grace M E Meers; Frank W Booth; Kevin L Fritsche; Christopher D Hardin; John P Thyfault; Jamal A Ibdah |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2012-08-16 |
Journal Detail:
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Title: American journal of physiology. Gastrointestinal and liver physiology Volume: 303 ISSN: 1522-1547 ISO Abbreviation: Am. J. Physiol. Gastrointest. Liver Physiol. Publication Date: 2012 Oct |
Date Detail:
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Created Date: 2012-10-16 Completed Date: 2013-01-11 Revised Date: 2013-04-16 |
Medline Journal Info:
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Nlm Unique ID: 100901227 Medline TA: Am J Physiol Gastrointest Liver Physiol Country: United States |
Other Details:
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Languages: eng Pagination: G979-92 Citation Subset: IM |
Affiliation:
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Department of Internal Medicine-Gastroenterology, University of Missouri, Columbia, Missouri, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apolipoproteins B / metabolism Fatty Acids / metabolism* Hepatocytes / metabolism* Lipid Metabolism Liver / metabolism* Mitochondria / metabolism Oxidation-Reduction RNA-Binding Proteins / genetics, metabolism* Rats Rats, Sprague-Dawley Transcription Factors / genetics, metabolism* Triglycerides / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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5T32 AR-48523-8/AR/NIAMS NIH HHS; DK-068210/DK/NIDDK NIH HHS; DK-088940/DK/NIDDK NIH HHS; R01 DK088940/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Apolipoproteins B; 0/Fatty Acids; 0/Ppargc1a protein, rat; 0/RNA-Binding Proteins; 0/Transcription Factors; 0/Triglycerides |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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