Document Detail


PGC-1α, a key modulator of p53, promotes cell survival upon metabolic stress.
MedLine Citation:
PMID:  22099309     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Metabolic stress results in p53 activation, which can trigger cell-cycle arrest, ROS clearance, or apoptosis. However, what determines the p53-mediated cell fate decision upon metabolic stress is not very well understood. We show here that PGC-1α binds to p53 and modulates its transactivation function, resulting in preferential transactivation of proarrest and metabolic target genes. Thus glucose starvation results in p53-dependent cell-cycle arrest and ROS clearance, but abrogation of PGC-1α expression results in extensive apoptosis. Additionally, prolonged starvation results in PGC-1α degradation concomitant with induction of apoptosis. We have also identified RNF2, a Polycomb group (PcG) protein, as the cognate E3 ubiquitin ligase. Starvation of mice where PGC-1α expression is abrogated results in loss of p53-mediated ROS clearance, enhanced p53-dependent apoptosis, and consequent severe liver atrophy. These findings provide key insights into the role of PGC-1α in regulating p53-mediated cell fate decisions in response to metabolic stress.
Authors:
Nirmalya Sen; Yatendra Kumar Satija; Sanjeev Das
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular cell     Volume:  44     ISSN:  1097-4164     ISO Abbreviation:  Mol. Cell     Publication Date:  2011 Nov 
Date Detail:
Created Date:  2011-11-21     Completed Date:  2012-01-16     Revised Date:  2012-03-06    
Medline Journal Info:
Nlm Unique ID:  9802571     Medline TA:  Mol Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  621-34     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Elsevier Inc. All rights reserved.
Affiliation:
Molecular Oncology Laboratory, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi 110067, India.
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis / drug effects*,  genetics
Cell Cycle / drug effects
Cell Line, Tumor
Cell Survival / drug effects,  genetics
Flow Cytometry
Gene Silencing / drug effects
Glucose / deficiency*
Humans
Liver / drug effects,  metabolism*,  pathology
Mice
Protein Binding
RNA, Small Interfering / pharmacology
Reactive Oxygen Species / metabolism
Repressor Proteins / genetics,  metabolism*
Reverse Transcriptase Polymerase Chain Reaction
Stress, Physiological / genetics*
Trans-Activators / antagonists & inhibitors,  genetics,  metabolism*
Transcriptional Activation / drug effects
Tumor Suppressor Protein p53 / genetics,  metabolism*
Chemical
Reg. No./Substance:
0/Ppargc1a protein, mouse; 0/RNA, Small Interfering; 0/Reactive Oxygen Species; 0/Repressor Proteins; 0/Trans-Activators; 0/Tumor Suppressor Protein p53; 50-99-7/Glucose; EC 6.3.2.-/Rnf2 protein, mouse
Comments/Corrections
Comment In:
Mol Cell. 2011 Nov 18;44(4):515-6   [PMID:  22099301 ]

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