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PE_PGRS30 is required for the full virulence of Mycobacterium tuberculosis.
MedLine Citation:
PMID:  22050772     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The role and function of PE_PGRS proteins of Mycobacterium tuberculosis (Mtb) remains elusive. In this study for the first time, Mtb isogenic mutants missing selected PE_PGRSs were used to investigate their role in the pathogenesis of tuberculosis (TB). We demonstrate that the MtbΔPE_PGRS30 mutant was impaired in its ability to colonize lung tissue and to cause tissue damage, specifically during the chronic steps of infection. Inactivation of PE_PGRS30 resulted in an attenuated phenotype in murine and human macrophages due to the inability of the Mtb mutant to inhibit phagosome-lysosome fusion. Using a series of functional deletion mutants of PE_PGRS30 to complement Mtb ΔPE_PGRS30, we show that the unique C-terminal domain of the protein is not required for the full virulence. Interestingly, when M. smegmatis recombinant strain expressing PE_PGRS30 was used to infect macrophages or mice in vivo, we observed enhanced cytotoxicity and cell death, and this effect was dependent upon the PGRS domain of the protein. Taken together these results indicate that PE_PGRS30 is necessary for the full virulence of Mtb and sufficient to induce cell death in host cells by the otherwise non pathogenic species M. smegmatis, clearly demonstrating that PE_PGRS30 is an Mtb virulence factor.
Authors:
Raffaella Iantomasi; Michela Sali; Alessandro Cascioferro; Ivana Palucci; Antonella Zumbo; Silvia Soldini; Stefano Rocca; Emanuela Greco; Giuseppe Maulucci; Marco De Spirito; Maurizio Fraziano; Giovanni Fadda; Riccardo Manganelli; Giovanni Delogu
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-11-3
Journal Detail:
Title:  Cellular microbiology     Volume:  -     ISSN:  1462-5822     ISO Abbreviation:  -     Publication Date:  2011 Nov 
Date Detail:
Created Date:  2011-11-4     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100883691     Medline TA:  Cell Microbiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2011 Blackwell Publishing Ltd.
Affiliation:
Institut of Microbiology, Institut of Physics, Universita' Cattolica del Sacro Cuore, L.go A. Gemelli, 8 - 00168 - Rome, Italy. Department of Histology, Microbiology and Medical Biotechnologies, University of Padua, Via A. Gabelli, 63 - 35121 - Padua, Italy; Department of Pathology and Veterinary Clinic, University of Sassari, Via Vienna - 07100 - Sassari, Italy. Department of Biology, University of Rome "Tor Vergata", 00133 Rome, Italy.
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