Document Detail


PEI-alginate nanocomposites as efficient in vitro gene transfection agents.
MedLine Citation:
PMID:  16891026     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The positive charge on PEI was partially shielded by forming ionic nanocomposites with a polysaccharide, alginic acid, in aqueous solution, bypassing tedious chemical synthesis. The content of alginic acid was varied systematically to obtain a series of nanocomposites. The nanocomposites were first characterized by assessing the surface charge (zeta potential), size (DLS) and morphology (AFM) followed by evaluation for their DNA interaction ability, cytotoxicity and transfection efficiency on various cell lines. The transfection efficiency of PEI-alginate (6.26%) nanocomposites improved dramatically (2-16-fold over native PEI) in all the cell lines studied. However, a decrease in transfection efficiency was observed on deviating from this optimal concentration of alginic acid in nanocomposites. Cytotoxicity of PEI-alginate/DNA complexes was nearly abolished on increasing the concentration of alginic acid in nanocomposites. PEI-alginate (6.26%) nanocomposites also delivered SiRNAs efficiently into mammalian cells, resulting in 80% suppression of GFP expression. The cellular uptake and endosomal escape of PEI-alginate nanocomposites and PEI were found to follow a similar route when transfection was carried out in presence of chloroquine, bafilomycin A1, cytochalasin B and methyl-beta-cyclodextrin. The results demonstrate a versatile vector that can be used for efficient cytoplasmic delivery of a broad range of nucleic acids.
Authors:
Soma Patnaik; Anita Aggarwal; Surendra Nimesh; Anita Goel; Munia Ganguli; Neeru Saini; Y Singh; K C Gupta
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-06-28
Journal Detail:
Title:  Journal of controlled release : official journal of the Controlled Release Society     Volume:  114     ISSN:  0168-3659     ISO Abbreviation:  J Control Release     Publication Date:  2006 Sep 
Date Detail:
Created Date:  2006-09-08     Completed Date:  2006-11-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8607908     Medline TA:  J Control Release     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  398-409     Citation Subset:  IM    
Affiliation:
Institute of Genomics and Integrative Biology, Mall Road, Delhi University Campus, Delhi-110 007, India.
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MeSH Terms
Descriptor/Qualifier:
Alginates / administration & dosage*
Animals
COS Cells
Cell Survival / drug effects
Cercopithecus aethiops
Cytochalasin B / pharmacology
DNA / administration & dosage*
Glucuronic Acid / administration & dosage
Hexuronic Acids / administration & dosage
Imines / administration & dosage*
Nanostructures*
Particle Size
Polyethylenes / administration & dosage*
RNA, Small Interfering / administration & dosage
Transfection / methods*
Chemical
Reg. No./Substance:
0/Alginates; 0/Hexuronic Acids; 0/Imines; 0/Polyethylenes; 0/RNA, Small Interfering; 0/poly(ethylene imine); 14930-96-2/Cytochalasin B; 576-37-4/Glucuronic Acid; 9005-32-7/alginic acid; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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