| PEGylation of protein-based MRI contrast agents improves relaxivities and biocompatibilities. | |
| | |
MedLine Citation:
|
PMID: 22178673 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Magnetic resonance imaging (MRI) has emerged as a leading diagnostic technique in clinical and preclinical settings. However, the application of MRI to assess specific disease markers for diagnosis and monitoring drug effect has been severely hampered by the lack of desired contrast agents with high relaxivities, and optimized in vivo retention time. We have reported the development of protein-based MRI contrast agents (ProCA1) by rational design of Gd(3+) binding sites into a stable protein resulting in significantly increased longitudinal (r(1)) and transverse (r(2)) relaxivities compared to Gd-DTPA. Here, we report a further improvement of protein contrast agents ProCA1 for in vivo imaging by protein modification with various sizes of polyethylene glycol (PEG) chain. PEGylation results in significant increases of both r(1) and r(2) relaxivities (up to 200%), and these high relaxivities persist even at field strengths up to 9.4T. In addition, our experimental results demonstrate that modified contrast agents have significant improvement of in vivo MR imaging and biocompatibilities including dose efficiency, protein solubility, blood retention time and decreased immunogenicity. Such improvement can be important to the animal imaging and pre-clinical research at high or ultra-high field where there is an urgent need for molecular imaging probes and optimized contrast agent. |
| | |
Authors:
|
Shunyi Li; Jie Jiang; Jin Zou; Jingjuan Qiao; Shenghui Xue; Lixia Wei; Robert Long; Liya Wang; Adriana Castiblanco; Natalie White; Jen Ngo; Hui Mao; Zhi-Ren Liu; Jenny J Yang |
Related Documents
:
|
16798013 - Functional pathway-defined mri diffusion measures reveal increased transverse diffusivi... 11746573 - Improving relative anisotropy measurement using directional correlation of diffusion te... 8210353 - The abdominal plain film. what will be its role in the future? |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-11-19 |
Journal Detail:
|
Title: Journal of inorganic biochemistry Volume: 107 ISSN: 1873-3344 ISO Abbreviation: - Publication Date: 2011 Nov |
Date Detail:
|
Created Date: 2011-12-19 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 7905788 Medline TA: J Inorg Biochem Country: - |
Other Details:
|
Languages: ENG Pagination: 111-118 Citation Subset: - |
Copyright Information:
|
Published by Elsevier Inc. |
Affiliation:
|
Department of Chemistry and Biology, Georgia State University, Atlanta, GA 30303, USA; College of Life Sciences, Hubei University, Wuhan, Hubei 430062, China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: DNA- and RNA-binding and enhanced DNA-photocleavage properties of a ferrocenyl-containing ruthenium(...
Next Document: Characterization of Bacillus thuringiensis isolates with potential for control of Aedes aegypti (Lin...