| PEG-liposomal oxaliplatin induces apoptosis in hunman colorectal cancer cells via Fas/FasL and caspase-8. | |
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MedLine Citation:
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PMID: 21888623 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Abstract Cellualr uptake of PEG-liposomal oxaliplatin induces bioactive changes in colorectal cancer, thus we investigated the apoptotic effect and anticancer mechanism of PEG-liposomal oxaliplatin. We treated the human colorectal cancer SW480 cell line with PEG-liposomal oxaliplatin and Caspase-8 inhibitor (Z-IETD-FMK). We evaluated apoptosis using flow cytometry (FCM) and Terminal deoxynucleotidyl transferase (TdT) mediated dUTP nick end labelling (TUNEL) assay. Expression of Fas/FasL and Cytochrome C was detected using FCM and Immunofluorescence assay. Expression of Caspase-8, Bid, Caspase-9, Caspase-7, and activated Caspase-3 (P17) was observed in the Western blot analyses. Our results indicated that PEG-liposomal oxaliplatin (containing 28 μg/ml oxaliplatin) markedly induced apoptosis in SW480 cells compared with 28 μg/ml free oxaliplatin. The expression levels of Fas, FasL, Cytochrome C, Caspase-9, Caspase-7, and activated Caspase-3 proteins were up-regulated with a corresponding increase in apoptosis, however, the expression levels of Caspase-8 and Bid were down-regulated as apoptosis increased. When cells were treated with Z-IETD-FMK, apoptosis was inhibited but there was little impact on the expression of Fas, FasL, Cytochrome C, Bid, Caspase-9, Caspase-7, and activated Caspase-3. These findings indicate that PEG-liposomal oxaliplatin enhanced the anticancer potency of the chemotherapeutic agent; moreover, Fas/FasL and Caspase-8 signaling pathways play a key role in mediating PEG-liposomal oxaliplatin-induced apoptosis. |
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Authors:
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Chuang Yang; Hai-Zhong Liu; Zhong-Xue Fu |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-9-2 |
Journal Detail:
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Title: Cell biology international Volume: - ISSN: 1095-8355 ISO Abbreviation: - Publication Date: 2011 Sep |
Date Detail:
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Created Date: 2011-9-5 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9307129 Medline TA: Cell Biol Int Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
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