Document Detail


PEA3 cooperates with c-Jun in regulation of HER2/neu transcription.
MedLine Citation:
PMID:  16786139     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
HER2/neu overexpressing breast tumors exhibit an increase in polyomavirus enhancer activator 3 (PEA3) expression. We examined the relationship between HER2/neu transcriptional activation and PEA3 in cooperation with c-Jun. HER2/neu promoter activity was decreased by deleting PEA3 binding site, and was downregulated when the PEA3 binding site was mutated. PEA3 and c-Jun each weakly enhanced luciferase expression of the HER2/neu promoter. However, the HER2/neu promoter response to PEA3 was considerably enhanced by c-Jun. Thus, we examined the interaction of PEA3 with c-Jun by the two-hybrid system, the transcriptional activity of PEA3 was specifically enhanced by c-Jun. When PEA3, c-Jun and coactivator p300 were cotransfected in MCF7 cells, the transcriptional activity of HER2/neu was increased by up to 20-fold. PEA3 and c-Jun-induced transcription of HER2/neu promoter was repressed by cotransfection of the dominant negative of p300. These results suggest that PEA3 and c-Jun stimulated synergistically the HER2/neu gene transcription with p300.
Authors:
Koshi Matsui; Kazuhito Sugimori; Hiraku Motomura; Naoko Ejiri; Kazuhiro Tsukada; Isao Kitajima
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Oncology reports     Volume:  16     ISSN:  1021-335X     ISO Abbreviation:  Oncol. Rep.     Publication Date:  2006 Jul 
Date Detail:
Created Date:  2006-06-20     Completed Date:  2006-11-09     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  9422756     Medline TA:  Oncol Rep     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  153-8     Citation Subset:  IM    
Affiliation:
Second Department of Surgery, University of Toyama, Toyama 930-0194, Japan.
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MeSH Terms
Descriptor/Qualifier:
Cell Line, Tumor
Gene Deletion
Gene Expression Regulation, Neoplastic*
Humans
Promoter Regions, Genetic
Protein Binding
Proto-Oncogene Proteins c-jun / metabolism*
Receptor, erbB-2 / metabolism,  physiology*
Transcription Factors / metabolism,  physiology*
Transcription, Genetic*
Transcriptional Activation
Transfection
Two-Hybrid System Techniques
p300-CBP Transcription Factors / metabolism
Chemical
Reg. No./Substance:
0/Proto-Oncogene Proteins c-jun; 0/Transcription Factors; 0/transcription factor PEA3; EC 2.3.1.48/p300-CBP Transcription Factors; EC 2.7.10.1/Receptor, erbB-2

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