Document Detail

PDGF-A controls mesoderm cell orientation and radial intercalation during Xenopus gastrulation.
MedLine Citation:
PMID:  21205800     Owner:  NLM     Status:  MEDLINE    
Radial intercalation is a common, yet poorly understood, morphogenetic process in the developing embryo. By analyzing cell rearrangement in the prechordal mesoderm during Xenopus gastrulation, we have identified a mechanism for radial intercalation. It involves cell orientation in response to a long-range signal mediated by platelet-derived growth factor (PDGF-A) and directional intercellular migration. When PDGF-A signaling is inhibited, prechordal mesoderm cells fail to orient towards the ectoderm, the endogenous source of PDGF-A, and no longer migrate towards it. Consequently, the prechordal mesoderm fails to spread during gastrulation. Orientation and directional migration can be rescued specifically by the expression of a short splicing isoform of PDGF-A, but not by a long matrix-binding isoform, consistent with a requirement for long-range signaling.
Erich W Damm; Rudolf Winklbauer
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Development (Cambridge, England)     Volume:  138     ISSN:  1477-9129     ISO Abbreviation:  Development     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-01-05     Completed Date:  2011-01-31     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8701744     Medline TA:  Development     Country:  England    
Other Details:
Languages:  eng     Pagination:  565-75     Citation Subset:  IM    
Department of Cell and Systems Biology, University of Toronto, 25 Harbord Street, Toronto, Ontario, Canada.
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MeSH Terms
Cell Movement / genetics,  physiology
Embryo, Nonmammalian / cytology*,  metabolism*,  ultrastructure
Gastrulation / genetics,  physiology*
In Situ Hybridization
Mesoderm / cytology*,  metabolism*,  ultrastructure
Microscopy, Electron, Scanning
Platelet-Derived Growth Factor / genetics,  metabolism*
Reverse Transcriptase Polymerase Chain Reaction
Signal Transduction / genetics,  physiology
Xenopus Proteins / genetics,  metabolism*
Grant Support
MOP-53075//Canadian Institutes of Health Research
Reg. No./Substance:
0/Platelet-Derived Growth Factor; 0/Xenopus Proteins; 0/platelet-derived growth factor A

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