| PDGF-BB enhances monocyte chemoattractant protein-1 mRNA stability in smooth muscle cells by downregulating ribonuclease activity. | |
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MedLine Citation:
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PMID: 16720030 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Platelet-derived growth factor (PDGF) has protean manifestations, including the regulation of growth and migration, in many cell types. We have previously reported that PDGF-BB induces the accumulation of monocyte chemoattractant protein (MCP)-1 mRNA in smooth muscle cells (SMC), in large part due to an increase in mRNA stability. To elucidate the mechanism by which PDGF-BB stabilizes MCP-1 mRNA, we have employed in vitro RNA gel mobility shift and decay assays. Cytoplasmic extracts from PDGF-BB-treated SMC increased the half-life of in vitro transcribed MCP-1 mRNA from approximately 45 min to >2 h. PDGF-BB-inhibitable degradation was not dependent on specific regions of the MCP-1 mRNA and was equally effective on a variety of in vitro transcribed mRNAs. Angiotensin II had a similar effect on MCP-1 mRNA stability, whereas tumor necrosis factor-alpha and basic fibroblast growth factor did not. The PDGF-BB-inhibitable RNAse activity was active at pH 6.6 and heat stable, but was sensitive to proteinase K. Extracts from PDGF-BB- or angiotensin II-treated cells inhibited the RNAse activity of control extracts, suggesting that the effect of PDGF-BB and angiotensin II are due to activation of a soluble inhibitor of the RNAse. The effect of PDGF-BB was blocked by inhibitors of tyrosine phosphorylation, but not by inhibitors of phosphatidylinositol 3-kinase or mitogen-activated protein kinases. These studies provide new insights into the mechanisms by which PDGF-BB enhances mRNA accumulation. |
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Authors:
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Bin Liu; Michael Poon; Mark B Taubman |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2006-05-23 |
Journal Detail:
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Title: Journal of molecular and cellular cardiology Volume: 41 ISSN: 0022-2828 ISO Abbreviation: J. Mol. Cell. Cardiol. Publication Date: 2006 Jul |
Date Detail:
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Created Date: 2006-07-03 Completed Date: 2006-10-27 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0262322 Medline TA: J Mol Cell Cardiol Country: England |
Other Details:
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Languages: eng Pagination: 160-9 Citation Subset: IM |
Affiliation:
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Department of Medicine, University of Rochester, Box 679, 601 Elmwood Avenue, Rochester, NY 14642, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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1-Phosphatidylinositol 3-Kinase
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metabolism Angiotensin II / pharmacology Animals Cells, Cultured Chemokine CCL2 / drug effects, genetics* Down-Regulation Enzyme Inhibitors / pharmacology Half-Life Isoenzymes / metabolism Male Mitogen-Activated Protein Kinases / antagonists & inhibitors, metabolism Muscle, Smooth, Vascular / drug effects*, metabolism Platelet-Derived Growth Factor / metabolism, pharmacology* Protein Kinase C / antagonists & inhibitors, metabolism RNA Stability / drug effects* RNA, Messenger / drug effects, metabolism Rats Rats, Sprague-Dawley Receptors, Platelet-Derived Growth Factor / metabolism Ribonucleases / drug effects, metabolism* src-Family Kinases / antagonists & inhibitors, metabolism |
| Grant Support | |
ID/Acronym/Agency:
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P01 77789//PHS HHS; R01 HL73364/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Chemokine CCL2; 0/Enzyme Inhibitors; 0/Isoenzymes; 0/Platelet-Derived Growth Factor; 0/RNA, Messenger; 0/platelet-derived growth factor BB; 11128-99-7/Angiotensin II; EC 2.7.1.-/PDGF receptor tyrosine kinase; EC 2.7.1.137/1-Phosphatidylinositol 3-Kinase; EC 2.7.1.137/phosphatidylinositol 3-kinase gamma; EC 2.7.10.1/Receptors, Platelet-Derived Growth Factor; EC 2.7.10.2/src-Family Kinases; EC 2.7.11.13/Protein Kinase C; EC 2.7.11.24/Mitogen-Activated Protein Kinases; EC 3.1.-/Ribonucleases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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