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PDE-5 and NOS II mRNA expression in menopausal women: a molecular biology study.
MedLine Citation:
PMID:  20177899     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
OBJECTIVE: Phosphodiesterase (PDE) and nitric oxide synthase (NOS), evaluated in male erectile dysfunction, are currently under study for their role in the female counterpart. We aim to assess PDE-5 and NOS II presence, at messenger Ribonucleic Acid (mRNA) level, in vaginal environment of menopausal women, by using molecular biology techniques.
METHODS: Specimens of vaginal tissue were obtained from 16 menopausal women undergoing surgery for pelvic organ prolapse. The two samples obtained for each patient, one under the urethra (called U) and one on the rest of the vaginal wall (called V), were tested for PDE-5 and NOS II by RT-PCR and by a densitometric semiquantitative analysis.
RESULTS: Of the V samples, 81.3% expressed PDE-5 and 100% NOS II. PDE-5 and NOS II expression were revealed in 87.5% of U specimens. A significant difference (P < 0.05) between V and U samples was found in the expression of NOS II (V vs. U: 24.14 vs. 7.25) and PDE-5 (V vs. U: 44.32 vs. 68.57).
CONCLUSIONS: Our results demonstrated the presence of PDE-5 and NOS II mRNA in periurethral and vaginal tissue of menopausal women. The distribution of PDE-5 and NOD II may indicate a physiologic role in the regulatory function of human vagina.
Authors:
Gianna Pace; Paola Palumbo; Gianfranca Miconi; Vania Silvestri; Maria Grazia Cifone; Carlo Vicentini
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Publication Detail:
Type:  Journal Article     Date:  2010-02-23
Journal Detail:
Title:  World journal of urology     Volume:  29     ISSN:  1433-8726     ISO Abbreviation:  World J Urol     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-03-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8307716     Medline TA:  World J Urol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  243-8     Citation Subset:  IM    
Affiliation:
Department of Surgical Sciences, University of L'Aquila, San Salvatore Street, Palace 6A, Coppito, 67100, L'Aquila, Italy, giannapace@gmail.com.
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