| PCR versus ELISA in diagnosis of human toxoplasmosis in Jeddah, Saudi Arabia. | |
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MedLine Citation:
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PMID: 19209756 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In this study, polymerase chain reaction (PCR) was compared with detection of specific antibodies to Toxoplasma by ELISA. A total of 70 blood samples from patients with different clinical features were tested, 43/70 (61.4%) were serologically Toxoplasma-IgG positive meanwhile only 16/43 (37.2%) were approved to be positive by PCR. A total of 22 samples (31.6%) were positive by PCR, but only 16 of them were positive by ELISA. So, Tox-IgG indicated catching Toxoplasma infection but not enough with immune modulation to determine acute case, while PCR besides, being valuable in diagnosis showed Toxoplasma parasitemia. |
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Authors:
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Abdulkader M D Tonkal |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of the Egyptian Society of Parasitology Volume: 38 ISSN: 1110-0583 ISO Abbreviation: J Egypt Soc Parasitol Publication Date: 2008 Dec |
Date Detail:
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Created Date: 2009-02-12 Completed Date: 2009-03-16 Revised Date: 2013-05-02 |
Medline Journal Info:
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Nlm Unique ID: 8102141 Medline TA: J Egypt Soc Parasitol Country: Egypt |
Other Details:
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Languages: eng Pagination: 707-14 Citation Subset: IM |
Affiliation:
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Department of Medical Parasitology, Faculty of Medicine King Abdul Aziz University, Jeddah, Saudi Arabia. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies, Protozoan / blood* DNA, Protozoan / analysis* Enzyme-Linked Immunosorbent Assay / methods*, standards Female Humans Immunocompromised Host Male Polymerase Chain Reaction / methods*, standards Pregnancy Pregnancy Complications, Parasitic / diagnosis Reproducibility of Results Saudi Arabia Sensitivity and Specificity Toxoplasma / immunology, isolation & purification* Toxoplasmosis / diagnosis* |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Protozoan; 0/DNA, Protozoan |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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