| A PCR-restriction fragment length polymorphism assay to genotype human metapneumovirus. | |
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MedLine Citation:
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PMID: 17986211 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Human metapneumovirus (hMPV) genotypes A and B show epidemiological and probably clinical differences. This report describes a fast and simple PCR-restriction fragment length polymorphism (PCR-RFLP) assay, involving digestion of the fusion protein gene with Tsp509I, that allows lineages A1, A2, B1 and B2 to be distinguished. The assay should help in elucidating the epidemiology of hMPV, and possibly in predicting the severity of clinical infection. |
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Authors:
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M Montes; D Vicente; O Esnal; G Cilla; E Pérez-Trallero |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-11-06 |
Journal Detail:
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Title: Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases Volume: 14 ISSN: 1198-743X ISO Abbreviation: Clin. Microbiol. Infect. Publication Date: 2008 Jan |
Date Detail:
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Created Date: 2007-12-24 Completed Date: 2008-02-14 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 9516420 Medline TA: Clin Microbiol Infect Country: France |
Other Details:
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Languages: eng Pagination: 91-3 Citation Subset: IM |
Affiliation:
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Servicio de Microbiología, Hospital Donostia, Basque Country University, San Sebastián, Spain. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amplified Fragment Length Polymorphism Analysis
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methods* Child Genotype Humans Metapneumovirus / classification*, genetics*, isolation & purification Molecular Epidemiology / methods Viral Fusion Proteins / genetics |
| Chemical | |
Reg. No./Substance:
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0/Viral Fusion Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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