| PCR primer evaluation for the detection of trypanosome DNA in naturally infected goats. | |
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MedLine Citation:
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PMID: 9870363 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The buffy coat of 76 roaming goats from the Bansang and Missira regions in Gambia, was examined for the presence of trypanosomes. From these animals, extractions from dry blood samples on filter paper were subjected to PCR using three different primer sets, ORPHON5J, GOL and TVW, specific for Trypanosoma brucei/Trypanosoma evansi, Trypanosoma congolense and Trypanosoma vivax, respectively. PCR results for T. congolense were 100% concordant with buffy coat examination. Besides the three T. vivax buffy coat-positive samples, another 15 yielded positive with the TVW primers. The ORPHON5J primers yielded no positive results. Analyses with the GOL primers of putatively negative samples, yielded aberrant band patterns whose diagnostic significance still remains to be determined. |
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Authors:
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P J Pereira de Almeida; M Ndao; B Goossens; S Osaer |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Veterinary parasitology Volume: 80 ISSN: 0304-4017 ISO Abbreviation: Vet. Parasitol. Publication Date: 1998 Dec |
Date Detail:
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Created Date: 1999-02-25 Completed Date: 1999-02-25 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7602745 Medline TA: Vet Parasitol Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 111-6 Citation Subset: IM |
Affiliation:
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Institute of Tropical Medicine, Antwerp, Belgium. palmeid@itg.be |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals DNA Primers / chemistry DNA, Protozoan / blood* Electrophoresis, Agar Gel / veterinary Gambia / epidemiology Goat Diseases / diagnosis*, epidemiology Goats Polymerase Chain Reaction / methods, veterinary* Sensitivity and Specificity Trypanosoma / genetics*, isolation & purification Trypanosomiasis, African / diagnosis, epidemiology, veterinary* |
| Chemical | |
Reg. No./Substance:
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0/DNA Primers; 0/DNA, Protozoan |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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