| PCR identification of bacteria in blood culture does not fit the daily workflow of a routine microbiology laboratory. | |
| | |
MedLine Citation:
|
PMID: 22170900 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
We have evaluated the GenoType blood culture assay (Hain Lifescience, Nehren, Germany) for the identification of bacteria in 233 positive blood cultures and assessed its suitability in the workflow of a routine microbiology laboratory. In 68/233 (29.2%) samples, the culture result could not be confirmed by the GenoType assay due to a lack of primers in the test, multiple organisms in the sample, or inconsistency with respect to the identification by culture. Although the GenoType blood culture assay gives satisfactory results for bacteria for which primers are available, there are difficulties in applying the test in the routine microbiology laboratory. |
| | |
Authors:
|
Santra Karumaa; Pauliina Kärpänoja; Hannu Sarkkinen |
Publication Detail:
|
Type: Comparative Study; Evaluation Studies; Journal Article Date: 2011-12-14 |
Journal Detail:
|
Title: Journal of clinical microbiology Volume: 50 ISSN: 1098-660X ISO Abbreviation: J. Clin. Microbiol. Publication Date: 2012 Mar |
Date Detail:
|
Created Date: 2012-02-20 Completed Date: 2012-05-04 Revised Date: 2012-09-27 |
Medline Journal Info:
|
Nlm Unique ID: 7505564 Medline TA: J Clin Microbiol Country: United States |
Other Details:
|
Languages: eng Pagination: 1031-3 Citation Subset: IM |
Affiliation:
|
Päijät-Häme Social and Health Care Group, Clinical Microbiology, Lahti, Finland. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Bacteremia
/
diagnosis*,
microbiology Bacteria / classification, genetics, isolation & purification* Bacteriological Techniques / methods* Blood / microbiology* DNA Primers / genetics Germany Humans Molecular Diagnostic Techniques / methods* Polymerase Chain Reaction / methods* Sensitivity and Specificity Workflow* |
| Chemical | |
Reg. No./Substance:
|
0/DNA Primers |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Epicardial and endocardial mapping determine most successful site of ablation for ventricular tachya...
Next Document: Evaluation of a recombinant antigen-based enzyme immunoassay for the diagnosis of noninvasive asperg...