Document Detail


PCR identification of Salmonella: potential contamination sources from production and postharvest handling of cantaloupes.
MedLine Citation:
PMID:  16786866     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Salmonella is one of the most frequently reported etiological agents in outbreaks of foodborne diseases associated with the consumption of cantaloupes. Sensitive and reliable methods for detecting and identifying foodborne microorganisms are needed. The PCR can be used to amplify specific DNA fragments and thus to detect and identify pathogenic bacteria. In this study, a PCR method was used to evaluate the incidence of Salmonella at cantaloupe production, harvest, and packaging steps, and the results were compared with those of the standard method for detection of Salmonella in foods (Mexican NOM-114-SSA1-1994). Salmonella was detected by both standard and PCR methods in 23.5% of the irrigation water samples but only by the PCR method in 9.1% of the groundwater samples, 4.8% of the chlorinated water samples, 16.7% of samples from the hands of packing workers, 20.6% of samples from the packed cantaloupes, and 25.7% of samples from the in-field cantaloupes. With the standard method, Salmonella was found in 8.3% of the crop soil samples. Statistical analysis indicated a significant difference in sensitivity (P < 0.05) between the two methods; the PCR method was 4.3 times more sensitive than the standard method. Salmonella was found at seven of the eight pointsevaluated during the production and postharvest handling of cantaloupe melons.
Authors:
I E Espinoza-Medina; F J Rodríguez-Leyva; I Vargas-Arispuro; M A Islas-Osuna; E Acedo-Félix; M A Martínez-Téllez
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of food protection     Volume:  69     ISSN:  0362-028X     ISO Abbreviation:  J. Food Prot.     Publication Date:  2006 Jun 
Date Detail:
Created Date:  2006-06-21     Completed Date:  2006-07-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7703944     Medline TA:  J Food Prot     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1422-5     Citation Subset:  IM    
Affiliation:
Coordinación de Tecnología de Alimentos de Origen Vegetal, Centro de Investigación en Alimentación y Desarrollo, A.C., A.P. 1735, 83000 Hermosillo, Sonora, México.
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MeSH Terms
Descriptor/Qualifier:
Consumer Product Safety
Cucumis melo / microbiology*
DNA, Bacterial / analysis*
Food Contamination / analysis*
Food Microbiology
Food Packaging / standards
Food-Processing Industry / standards
Gene Amplification
Polymerase Chain Reaction / methods*
Salmonella / isolation & purification*
Sensitivity and Specificity
Chemical
Reg. No./Substance:
0/DNA, Bacterial

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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