Document Detail


PCR identification of Leishmania in diagnosis and control of canine Leishmaniasis.
MedLine Citation:
PMID:  17196339     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Leishmaniases are endemic in many countries, mainly in rural areas. In Brazil, Leishmania infection is responsible for many cases of Leishmaniases, including recent reports in urban regions. Despite their sensitivity, traditional serological and parasitological methods for detecting Leishmaniases have proven inadequate for species discrimination. This study aimed to identify Leishmania species in biological samples by a fast methodology, avoiding "in vitro" cultivation. Knowledge of the Leishmania species is an important tool in regions where both New World visceral leishmaniasis (VL) and cutaneous leishmaniasis (CL) are prevalent. As these new foci appear in areas not traditionally endemic for VL, the main problem is to distinguish between true autochthonous infections and infections acquired in other well-known endemic areas. Since, domestic dogs are known to be the main VL and CL reservoir, they are regularly investigated in endemic areas to prevent, principally, severe and often fatal VL in humans. However, several infected dogs present no clinical signs or clinical signs similar to other canine diseases. Here, we evaluated the ability of PCR to diagnose VL and distinguish L. (L.) chagasi from other Leishmania species in domestic dogs. Samples from 114 dogs from 30 cities (Sao Paulo, Brazil) were divided into two groups: 44 symptomatic and 70 asymptomatic. They were assayed by parasitological methods (culture and microscopic examination) and PCR to determine L. (L.) chagasi, L. (V.) braziliensis; and in some cases, Leishmania spp. Parasitological tests and PCR-L. chagasi were concordant in 105 samples (92%). VL was confirmed in 49 dogs, while 56 had negative results. Of the 114 samples, 9 had discordant results, but were further tested by PCR-Leishmania spp. with positive results. VL was also confirmed in 4 dogs having negative parasitological tests and positive PCR-L. chagasi. Consequently, this PCR was positive for 100% (53/49) of dogs with parasites detected in parasitological tests. Also, PCR demonstrated high specificity detecting 61 dogs negative for VL. Leishmania infection was negative in 56 dogs, and 5 with positive culture and PCR-Leishmania spp. had CL since they were positive in PCR-L. braziliensis. This study shows the importance of including PCR in diagnosis of Leishmaniases by differential diagnosis contributing to the surveillance and control of VL programs.
Authors:
Aparecida H S Gomes; Isabelle M R Ferreira; Maria L S R Lima; Elaine A Cunha; Andrea S Garcia; Maria F L Araújo; Vera L Pereira-Chioccola
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-12-28
Journal Detail:
Title:  Veterinary parasitology     Volume:  144     ISSN:  0304-4017     ISO Abbreviation:  Vet. Parasitol.     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-02-20     Completed Date:  2007-05-18     Revised Date:  2007-11-08    
Medline Journal Info:
Nlm Unique ID:  7602745     Medline TA:  Vet Parasitol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  234-41     Citation Subset:  IM    
Affiliation:
Laboratorio de Parasitologia, Instituto Adolfo Lutz, Av. Dr Arnaldo, CEP 01246-902, São Paulo, SP, Brazil.
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MeSH Terms
Descriptor/Qualifier:
Animals
DNA, Protozoan
Dog Diseases / diagnosis,  epidemiology,  parasitology*
Dogs
Leishmania / classification,  genetics*,  isolation & purification*
Leishmaniasis, Visceral / diagnosis,  epidemiology,  parasitology,  veterinary*
Mediterranean Region / epidemiology
Polymerase Chain Reaction / methods,  veterinary*
Sensitivity and Specificity
Species Specificity
Time Factors
Chemical
Reg. No./Substance:
0/DNA, Protozoan
Comments/Corrections
Erratum In:
Vet Parasitol. 2007 Nov 10;149(3-4):298

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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