| PCR detection of psychrotolerant clostridia associated with deep tissue spoilage of vacuum-packed chilled meats. | |
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MedLine Citation:
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PMID: 12390498 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIMS: To develop a practical molecular procedure that directly (without isolation) and specifically detects the presence of clostridia, which cause the deep tissue spoilage condition. METHODS AND RESULTS: A primer set was designed and a PCR amplification procedure developed to detect the presence of Clostridium algidicarnis and Cl. putrefaciens 16S rDNA gene fragments in meat. The procedure yielded amplicons of the expected size with homologous DNA templates, but failed to give PCR products with DNAs from 47 food clostridia and common meat spoilage micro-organisms. The minimum level of detection was 10(4) cfu g-1 for nonenriched meat samples. Based on the established specificity of these primers, as well as DNA sequencing of amplicons, the presence of Cl. algidicarnis and/or Cl. putrefaciens was confirmed in a swab sample taken from the cartilage of an ovine stifle joint, which on opening exhibited strong offensive odours. CONCLUSIONS: The developed method can be used for rapid detection of clostridia causing deep tissue spoilage in commercial vacuum packs. SIGNIFICANCE AND IMPACT OF THE STUDY: The paper reports practical procedures that can be used for rapid confirmation of the causative agents of deep tissue clostridial spoilage in commercial vacuum-packed chilled meats. |
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Authors:
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J A Boerema; D M Broda; R G Bell |
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Publication Detail:
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Type: Evaluation Studies; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Letters in applied microbiology Volume: 35 ISSN: 0266-8254 ISO Abbreviation: Lett. Appl. Microbiol. Publication Date: 2002 |
Date Detail:
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Created Date: 2002-10-22 Completed Date: 2003-01-27 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 8510094 Medline TA: Lett Appl Microbiol Country: England |
Other Details:
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Languages: eng Pagination: 446-50 Citation Subset: IM |
Affiliation:
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AgResearch, Hamilton, New Zealand. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Clostridium / genetics, growth & development, isolation & purification* Cold Temperature* DNA, Bacterial / analysis DNA, Ribosomal / analysis Food Microbiology* Food Packaging* Meat Products / microbiology* Polymerase Chain Reaction* / methods Sequence Analysis, DNA Sheep Vacuum |
| Chemical | |
Reg. No./Substance:
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0/DNA, Bacterial; 0/DNA, Ribosomal |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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