| PCR-based methodology for detecting multidrug-resistant strains of Mycobacterium tuberculosis Beijing family circulating in Russia. | |
| | |
MedLine Citation:
|
PMID: 12783278 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The Beijing genotype of Mycobacterium tuberculosis has been identified in 40-50% of the clinical isolates studied in Russia during the last decade. This genotype has been reported to be associated with multiple drug resistance and possesses some significant pathogenic properties. Therefore, early identification of such strains is of extreme importance in the timely detection of drug resistance. The present study was performed on 354 strains isolated in Russia from 1996 to 2002 and previously characterised by IS 6110-restriction fragment length polymorphism (RFLP) typing and spoligotyping. These strains included 198 Beijing family strains and 156 strains of other genotypes (IS 6110-RFLP profiles). A subsequent polymerase chain reaction (PCR) analysis with IS 6110-derived outwardly oriented primers (IS 6110-PCR) easily discriminated the Beijing strains from non-Beijing strains. The multiplex allele-specific (MAS)-PCR assays were further used to detect mutations in katG315 and rpoB531, associated with resistance to isoniazid and rifampin, respectively. The katG315 and rpoB531 mutations were found to be more prevalent among Beijing (96.8% and 77.3%) than among non-Beijing strains (85.7% and 28%). Consequently, we propose a two-step methodology based on routine PCR and simple agarose gel electrophoresis in order to detect (i) a Beijing family strain using IS 6110-PCR, and, (ii) its possible resistance to the major anti-tuberculosis drugs using specific MAS-PCR assays. |
| | |
Authors:
|
I Mokrousov; T Otten; A Vyazovaya; E Limeschenko; M L Filipenko; C Sola; N Rastogi; L Steklova; B Vyshnevskiy; O Narvskaya |
Related Documents
:
|
2566378 - Correlation between reversing of multidrug resistance and inhibiting of [3h]azidopine p... 8203018 - Multidrug resistance pumps in bacteria: variations on a theme. 14673768 - Effect of drug resistance on the generation of secondary cases of tuberculosis. 10322098 - Multidrug-resistant tuberculosis in alberta and british columbia, 1989 to 1998. 8160348 - Antigenic differences within actinobacillus pleuropneumoniae serotype 1. 6788838 - The relationship of two equine mycoplasmas to mycoplasma mycoides. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2003-06-03 |
Journal Detail:
|
Title: European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology Volume: 22 ISSN: 0934-9723 ISO Abbreviation: Eur. J. Clin. Microbiol. Infect. Dis. Publication Date: 2003 Jun |
Date Detail:
|
Created Date: 2003-06-23 Completed Date: 2003-09-10 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 8804297 Medline TA: Eur J Clin Microbiol Infect Dis Country: Germany |
Other Details:
|
Languages: eng Pagination: 342-8 Citation Subset: IM |
Affiliation:
|
Laboratory of Molecular Microbiology, St. Petersburg Pasteur Institute, 14 Mira Street, 197101 St. Petersburg, Russia. imokrousov@mail.ru |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Antitubercular Agents
/
pharmacology* Base Sequence DNA Fingerprinting DNA, Bacterial / analysis Female Genotype Humans Male Microbial Sensitivity Tests Molecular Sequence Data Mutation* Mycobacterium tuberculosis / drug effects*, genetics* Polymerase Chain Reaction / methods Polymorphism, Restriction Fragment Length Russia / epidemiology Sensitivity and Specificity Tuberculosis, Multidrug-Resistant / drug therapy, epidemiology, genetics* |
| Chemical | |
Reg. No./Substance:
|
0/Antitubercular Agents; 0/DNA, Bacterial |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Four-year experience of use of the Cobas Amplicor system for rapid detection of Mycobacterium tuberc...
Next Document: Bacteremic pneumococcal cellulitis compared with bacteremic cellulitis caused by Staphylococcus aure...